6ibp: Difference between revisions

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-'''Unreleased structure'''
-The entry 6ibp is ON HOLD
+==Structure of a psychrophilic CCA-adding enzyme at room temperature in ChipX microfluidic device==
+<StructureSection load='6ibp' size='340' side='right'caption='[[6ibp]], [[Resolution|resolution]] 2.54&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6ibp]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IBP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IBP FirstGlance]. <br>
+</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/CCA_tRNA_nucleotidyltransferase CCA tRNA nucleotidyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.72 2.7.7.72] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ibp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ibp OCA], [http://pdbe.org/6ibp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ibp RCSB], [http://www.ebi.ac.uk/pdbsum/6ibp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ibp ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/A0A1C7DQ98_9BACL A0A1C7DQ98_9BACL]] Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate.[SAAS:SAAS00711016]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Determining optimal conditions for the production of well diffracting crystals is a key step in every biocrystallography project. Here, a microfluidic device is described that enables the production of crystals by counter-diffusion and their direct on-chip analysis by serial crystallography at room temperature. Nine 'non-model' and diverse biomacromolecules, including seven soluble proteins, a membrane protein and an RNA duplex, were crystallized and treated on-chip with a variety of standard techniques including micro-seeding, crystal soaking with ligands and crystal detection by fluorescence. Furthermore, the crystal structures of four proteins and an RNA were determined based on serial data collected on four synchrotron beamlines, demonstrating the general applicability of this multipurpose chip concept.
-Authors: de Wijn, R., Hennig, O., Rollet, K., Bluhm, A., Betat, H., Moerl, M., Lorber, B., Sauter, C.
+A simple and versatile microfluidic device for efficient biomacromolecule crystallization and structural analysis by serial crystallography.,de Wijn R, Hennig O, Roche J, Engilberge S, Rollet K, Fernandez-Millan P, Brillet K, Betat H, Morl M, Roussel A, Girard E, Mueller-Dieckmann C, Fox GC, Olieric V, Gavira JA, Lorber B, Sauter C IUCrJ. 2019 Apr 19;6(Pt 3):454-464. doi: 10.1107/S2052252519003622. eCollection, 2019 May 1. PMID:31098026<ref>PMID:31098026</ref>
-Description: Structure of a psychrophilic CCA-adding enzyme at room temperature in ChipX microfluidic device
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6ibp" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: CCA tRNA nucleotidyltransferase]]
+[[Category: Large Structures]]
+[[Category: Betat, H]]
 [[Category: Bluhm, A]]
+[[Category: Hennig, O]]
+[[Category: Lorber, B]]
 [[Category: Moerl, M]]
+[[Category: Rollet, K]]
 [[Category: Sauter, C]]
-[[Category: Lorber, B]]
+[[Category: Wijn, R de]]
-[[Category: Betat, H]]
+[[Category: Chipx]]
-[[Category: Hennig, O]]
+[[Category: Psychrophilic enzyme]]
-[[Category: Rollet, K]]
+[[Category: Rna binding protein]]
-[[Category: De Wijn, R]]
+[[Category: Trna maturation]]
+[[Category: Trna nucleotidyltransferase]]