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==Crystal structure of P. aeruginosa OXA10== | ==Crystal structure of P. aeruginosa OXA10== | ||
<StructureSection load='4wz5' size='340' side='right' caption='[[4wz5]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='4wz5' size='340' side='right'caption='[[4wz5]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4wz5]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WZ5 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4wz5]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WZ5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WZ5 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3VU:{(3R)-6-[(3-AMINO-1,2,4-THIADIAZOL-5-YL)OXY]-1-HYDROXY-4,5-DIMETHYL-1,3-DIHYDRO-2,1-BENZOXABOROL-3-YL}ACETIC+ACID'>3VU</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3VU:{(3R)-6-[(3-AMINO-1,2,4-THIADIAZOL-5-YL)OXY]-1-HYDROXY-4,5-DIMETHYL-1,3-DIHYDRO-2,1-BENZOXABOROL-3-YL}ACETIC+ACID'>3VU</scene>, <scene name='pdbligand=CO2:CARBON+DIOXIDE'>CO2</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=KCX:LYSINE+NZ-CARBOXYLIC+ACID'>KCX</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wyy|4wyy]], [[4wz4|4wz4]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4wyy|4wyy]], [[4wz4|4wz4]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bla, oxa10, pse2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-lactamase Beta-lactamase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.5.2.6 3.5.2.6] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wz5 OCA], [http://pdbe.org/4wz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4wz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wz5 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4wz5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4wz5 OCA], [http://pdbe.org/4wz5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4wz5 RCSB], [http://www.ebi.ac.uk/pdbsum/4wz5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4wz5 ProSAT]</span></td></tr> | ||
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</div> | </div> | ||
<div class="pdbe-citations 4wz5" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 4wz5" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Beta-lactamase]] | [[Category: Beta-lactamase]] | ||
[[Category: Large Structures]] | |||
[[Category: Ferguson, A D]] | [[Category: Ferguson, A D]] | ||
[[Category: Beta lactamase]] | [[Category: Beta lactamase]] | ||
[[Category: Hydrolase-hydrolase inhibitor complex]] | [[Category: Hydrolase-hydrolase inhibitor complex]] | ||
[[Category: Inhibtor]] | [[Category: Inhibtor]] | ||
Revision as of 12:13, 23 May 2019
Crystal structure of P. aeruginosa OXA10Crystal structure of P. aeruginosa OXA10
Structural highlights
Function[BLO10_PSEAI] Hydrolyzes both carbenicillin and oxacillin. Publication Abstract from PubMedBacterially expressed beta-lactamases are rapidly eroding the clinical utility of the important beta-lactam class of antibacterials, significantly impairing our ability to fight serious bacterial infections. This paper describes a study of oxaborole-derived beta-lactamase inhibitors in which crystal structures and computational modeling aided in the rational design of analogues with improved spectrum of activity against class A, C, and D enzymes. Crystal structures of two of these inhibitors covalently bound to two different serine beta-lactamases, class C Pseudomonas aeruginosa AmpC and class D OXA-10, are described herein. Improved physicochemical properties as well as increased activity against an array of beta-lactamases resulted in substantial restoration of susceptibility to ceftazidime in Escherichia coli and Klebsiella pneumoniae. 4,5-Disubstituted 6-Aryloxy-1,3-dihydrobenzo[c][1,2]oxaboroles Are Broad-Spectrum Serine beta-Lactamase Inhibitors.,McKinney DC, Zhou F, Eyermann CJ, Ferguson AD, Prince DB, Breen J, Giacobbe RA, Lahiri S, Verheijen JC ACS Infect Dis. 2015 Jul 10;1(7):310-6. doi: 10.1021/acsinfecdis.5b00031. Epub, 2015 Jun 18. PMID:27622821[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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