4v4m: Difference between revisions
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==1.45 Angstrom Structure of STNV coat protein== | ==1.45 Angstrom Structure of STNV coat protein== | ||
<StructureSection load='4v4m' size='340' side='right' caption='[[4v4m]], [[Resolution|resolution]] 1.45Å' scene=''> | <StructureSection load='4v4m' size='340' side='right'caption='[[4v4m]], [[Resolution|resolution]] 1.45Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4v4m]] is a 60 chain structure. This structure supersedes | <table><tr><td colspan='2'>[[4v4m]] is a 60 chain structure with sequence from [http://en.wikipedia.org/wiki/Stnv1 Stnv1]. This structure supersedes the now removed PDB entries [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=1vtz 1vtz] and [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=3rqv 3rqv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4V4M OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4V4M FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3s4g|3s4g]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3s4g|3s4g]]</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4m OCA], [http://www.rcsb.org/pdb/explore.do?structureId=4v4m RCSB], [http://www.ebi.ac.uk/pdbsum/4v4m PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4v4m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4v4m OCA], [http://pdbe.org/4v4m PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4v4m RCSB], [http://www.ebi.ac.uk/pdbsum/4v4m PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4v4m ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
</div> | </div> | ||
<div class="pdbe-citations 4v4m" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Virus coat protein|Virus coat protein]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Stnv1]] | |||
[[Category: Dennis, C A]] | [[Category: Dennis, C A]] | ||
[[Category: Lane, C L]] | [[Category: Lane, C L]] | ||
Revision as of 12:04, 23 May 2019
1.45 Angstrom Structure of STNV coat protein1.45 Angstrom Structure of STNV coat protein
Structural highlights
Publication Abstract from PubMedA codon-optimised gene has been expressed in Escherichia coli to produce the coat protein (CP) of the Satellite Tobacco Necrosis Virus. This protein assembles in vivo into capsids closely resembling those of the T=1 wild-type virus. These virus-like particles (VLPs) package the recombinant mRNA transcript and can be disassembled and reassembled using different buffer conditions. The X-ray crystal structure of the VLP has been solved and refined at 1.4 A resolution and shown to be very similar to that of wild-type Satellite Tobacco Necrosis Virus, except that icosahedral symmetry constraints could be removed to reveal differences between subunits, presumably owing to crystal packing. An additional low-resolution X-ray crystal structure determination revealed well-ordered RNA fragments lodged near the inside surface of the capsid, close to basic clusters formed by the N-terminal helices that project into the interior of the particle. The RNA consists of multiple copies of a 3-bp helical stem, with a single unpaired base at the 3' end, and probably consists of a number of short stem-loops where the loop region is disordered. The arrangement of the RNA is different from that observed in other satellite viruses. Construction and Crystal Structure of Recombinant STNV Capsids.,Lane SW, Dennis CA, Lane CL, Trinh CH, Rizkallah PJ, Stockley PG, Phillips SE J Mol Biol. 2011 Oct 14;413(1):41-50. Epub 2011 Aug 3. PMID:21839089[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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