4weu: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Co-complex structure of the F4 fimbrial adhesin FaeG variant ad with llama single domain antibody V3== | ==Co-complex structure of the F4 fimbrial adhesin FaeG variant ad with llama single domain antibody V3== | ||
<StructureSection load='4weu' size='340' side='right' caption='[[4weu]], [[Resolution|resolution]] 2.61Å' scene=''> | <StructureSection load='4weu' size='340' side='right'caption='[[4weu]], [[Resolution|resolution]] 2.61Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4weu]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WEU FirstGlance]. <br> | <table><tr><td colspan='2'>[[4weu]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895] and [http://en.wikipedia.org/wiki/Camelus_glama Camelus glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4WEU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4WEU FirstGlance]. <br> | ||
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hlr|3hlr]]</td></tr> | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3hlr|3hlr]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">faeG ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4weu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4weu OCA], [http://pdbe.org/4weu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4weu RCSB], [http://www.ebi.ac.uk/pdbsum/4weu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4weu ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4weu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4weu OCA], [http://pdbe.org/4weu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4weu RCSB], [http://www.ebi.ac.uk/pdbsum/4weu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4weu ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| Line 20: | Line 21: | ||
==See Also== | ==See Also== | ||
*[[Antibody 3D structures|Antibody 3D structures]] | |||
*[[Pilin|Pilin]] | *[[Pilin|Pilin]] | ||
== References == | == References == | ||
| Line 25: | Line 27: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus coli migula 1895]] | |||
[[Category: Camelus glama]] | |||
[[Category: Large Structures]] | |||
[[Category: Broeck, I Van den]] | [[Category: Broeck, I Van den]] | ||
[[Category: Greve, H De]] | [[Category: Greve, H De]] | ||
Revision as of 11:44, 23 May 2019
Co-complex structure of the F4 fimbrial adhesin FaeG variant ad with llama single domain antibody V3Co-complex structure of the F4 fimbrial adhesin FaeG variant ad with llama single domain antibody V3
Structural highlights
Function[FAEG3_ECOLX] K88 major fimbrial subunit. Fimbriae (also called pili), are polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell. They enable bacteria to colonize the epithelium of specific host organs. Publication Abstract from PubMedEnterotoxigenic Escherichia coli that cause neonatal and post-weaning diarrhea in piglets express F4 fimbriae to mediate attachment towards host receptors. Recently we described how llama single domain antibodies (VHHs) fused to IgA, produced in Arabidopsis thaliana seeds and fed to piglets resulted in a progressive decline in shedding of F4 positive ETEC bacteria. Here we present the structures of these inhibiting VHHs in complex with the major adhesive subunit FaeG. A conserved surface, distant from the lactose binding pocket, is targeted by these VHHs, highlighting the possibility of targeting epitopes on single-domain adhesins that are non-involved in receptor binding. Structural insight in the inhibition of adherence of F4 fimbriae producing enterotoxigenic Escherichia coli by llama single domain antibodies.,Moonens K, Van den Broeck I, Okello E, Pardon E, De Kerpel M, Remaut H, De Greve H Vet Res. 2015 Feb 24;46(1):14. doi: 10.1186/s13567-015-0151-x. PMID:25828907[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
|
| ||||||||||||||||||