6dkq: Difference between revisions
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==Crystal structure of the Shr Hemoglobin Interacting Domain 2== | ==Crystal structure of the Shr Hemoglobin Interacting Domain 2== | ||
<StructureSection load='6dkq' size='340' side='right' caption='[[6dkq]], [[Resolution|resolution]] 1.50Å' scene=''> | <StructureSection load='6dkq' size='340' side='right'caption='[[6dkq]], [[Resolution|resolution]] 1.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6dkq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DKQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[6dkq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_scarlatinae"_klein_1884 "micrococcus scarlatinae" klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DKQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DKQ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">shr ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1314 "Micrococcus scarlatinae" Klein 1884])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dkq OCA], [http://pdbe.org/6dkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dkq RCSB], [http://www.ebi.ac.uk/pdbsum/6dkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dkq ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dkq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dkq OCA], [http://pdbe.org/6dkq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dkq RCSB], [http://www.ebi.ac.uk/pdbsum/6dkq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dkq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In order to proliferate and mount an infection, many bacterial pathogens need to acquire iron from their host. The most abundant iron source in the body is the oxygen transporter hemoglobin (Hb). Streptococcus pyogenes, a potentially lethal human pathogen, uses the Shr protein to capture Hb on the cell surface. Shr is an important virulence factor, yet the mechanism by which it captures Hb and acquires its heme is not well-understood. Here, we show using NMR and biochemical methods that Shr binds Hb using two related modules that were previously defined as domains of unknown function (DUF1533). These hemoglobin-interacting domains (HIDs), called HID1 and HID2, are autonomously folded and independently bind Hb. The 1.5 A resolution crystal structure of HID2 revealed that it is a structurally unique Hb-binding domain. Mutagenesis studies revealed a conserved tyrosine in both HIDs that is essential for Hb binding. Our biochemical studies indicate that HID2 binds Hb with higher affinity than HID1 and that the Hb tetramer is engaged by two Shr receptors. NMR studies reveal the presence of a third autonomously folded domain between HID2 and a heme-binding NEAT1 domain, suggesting that this linker domain may position NEAT1 near Hb for heme capture. | |||
The Streptococcus pyogenes Shr protein captures human hemoglobin using two structurally unique binding domains.,Macdonald R, Cascio D, Collazo MJ, Phillips M, Clubb RT J Biol Chem. 2018 Nov 23;293(47):18365-18377. doi: 10.1074/jbc.RA118.005261. Epub, 2018 Oct 9. PMID:30301765<ref>PMID:30301765</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6dkq" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Micrococcus scarlatinae klein 1884]] | |||
[[Category: Large Structures]] | |||
[[Category: Cascio, D]] | [[Category: Cascio, D]] | ||
[[Category: Clubb, R T]] | [[Category: Clubb, R T]] | ||