2fav: Difference between revisions

Revision as of 08:19, 13 April 2008

Crystal structure of SARS macro domain in complex with ADP-ribose at 1.8 A resolution

OverviewOverview

Macro domains constitute a protein module family found associated with specific histones and proteins involved in chromatin metabolism. In addition, a small number of animal RNA viruses, such as corona- and toroviruses, alphaviruses, and hepatitis E virus, encode macro domains for which, however, structural and functional information is extremely limited. Here, we characterized the macro domains from hepatitis E virus, Semliki Forest virus, and severe acute respiratory syndrome coronavirus (SARS-CoV). The crystal structure of the SARS-CoV macro domain was determined at 1.8-Angstroms resolution in complex with ADP-ribose. Information derived from structural, mutational, and sequence analyses suggests a close phylogenetic and, most probably, functional relationship between viral and cellular macro domain homologs. The data revealed that viral macro domains have relatively poor ADP-ribose 1"-phosphohydrolase activities (which were previously proposed to be their biologically relevant function) but bind efficiently free and poly(ADP-ribose) polymerase 1-bound poly(ADP-ribose) in vitro. Collectively, these results suggest to further evaluate the role of viral macro domains in host response to viral infection.

About this StructureAbout this Structure

2FAV is a Single protein structure of sequence from Human sars coronavirus. Full crystallographic information is available from OCA.

ReferenceReference

Structural and functional basis for ADP-ribose and poly(ADP-ribose) binding by viral macro domains., Egloff MP, Malet H, Putics A, Heinonen M, Dutartre H, Frangeul A, Gruez A, Campanacci V, Cambillau C, Ziebuhr J, Ahola T, Canard B, J Virol. 2006 Sep;80(17):8493-502. PMID:16912299 Page seeded by OCA on Sun Apr 13 08:19:01 2008

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OCA

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-}}
 '''Crystal structure of SARS macro domain in complex with ADP-ribose at 1.8 A resolution'''
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 [[Category: MSGP, Marseilles Structural Genomics Program.@.AFMB.]]
 [[Category: Malet, H.]]
-[[Category: marseilles structural genomics program @ afmb]]
+[[Category: Marseilles structural genomics program @ afmb]]
-[[Category: msgp]]
+[[Category: Msgp]]
-[[Category: protein-adp-ribose complex]]
+[[Category: Protein-adp-ribose complex]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: structural proteomics in europe]]
+[[Category: Structural proteomics in europe]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:19:01 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:00:12 2008''