2bt1: Difference between revisions

Revision as of 08:18, 13 April 2008

EPSTEIN BARR VIRUS DUTPASE IN COMPLEX WITH A,B-IMINO DUTP

OverviewOverview

Deoxyuridine 5'-triphosphate pyrophosphatases (dUTPases) are ubiquitous enzymes cleaving dUTP into dUMP and pyrophosphate. They occur as monomeric, dimeric, or trimeric molecules. The trimeric and monomeric enzymes both contain the same five characteristic sequence motifs but in a different order, whereas the dimeric enzymes are not homologous. Monomeric dUTPases only occur in herpesviruses, such as Epstein-Barr virus (EBV). Here, we describe the crystal structures of EBV dUTPase in complex with the product dUMP and a substrate analog alpha,beta-imino-dUTP. The molecule consists of three domains forming one active site that has a structure extremely similar to one of the three active sites of trimeric dUTPases. The three domains functionally correspond to the subunits of the trimeric form. Domains I and II have the dUTPase fold, but they differ considerably in the regions that are not involved in the formation of the unique active site, whereas domain III has only little secondary structure.

About this StructureAbout this Structure

2BT1 is a Single protein structure of sequence from Human herpesvirus 4. Full crystallographic information is available from OCA.

ReferenceReference

The monomeric dUTPase from Epstein-Barr virus mimics trimeric dUTPases., Tarbouriech N, Buisson M, Seigneurin JM, Cusack S, Burmeister WP, Structure. 2005 Sep;13(9):1299-310. PMID:16154087 Page seeded by OCA on Sun Apr 13 08:18:29 2008

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
 [[Image:2bt1.gif|left|200px]]
- {{Structure
+<!--
-|PDB= 2bt1 |SIZE=350|CAPTION= <scene name='initialview01'>2bt1</scene>, resolution 2.70&Aring;
+The line below this paragraph, containing "STRUCTURE_2bt1", creates the "Structure Box" on the page.
-|SITE= <scene name='pdbsite=AC1:Mg+Binding+Site+For+Chain+A'>AC1</scene>
+You may change the PDB parameter (which sets the PDB file loaded into the applet)
-|LIGAND= <scene name='pdbligand=DUP:2&#39;-DEOXYURIDINE+5&#39;-ALPHA,BETA-IMIDO-TRIPHOSPHATE'>DUP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>
+or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/dUTP_diphosphatase dUTP diphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.6.1.23 3.6.1.23] </span>
+or leave the SCENE parameter empty for the default display.
-|GENE=
+-->
-|DOMAIN=
+{{STRUCTURE_2bt1|  PDB=2bt1  |  SCENE=  }}
-|RELATEDENTRY=
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bt1 OCA], [http://www.ebi.ac.uk/pdbsum/2bt1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2bt1 RCSB]</span>
-}}
 '''EPSTEIN BARR VIRUS DUTPASE IN COMPLEX WITH A,B-IMINO DUTP'''
@@ Line 25: / Line 22: @@
 [[Category: Human herpesvirus 4]]
 [[Category: Single protein]]
-[[Category: dUTP diphosphatase]]
+[[Category: DUTP diphosphatase]]
 [[Category: Buisson, M.]]
 [[Category: Burmeister, W P.]]
@@ Line 32: / Line 29: @@
 [[Category: Seigneurin, J M.]]
 [[Category: Tarbouriech, N.]]
-[[Category: dutpase]]
+[[Category: Dutpase]]
-[[Category: hydrolase]]
+[[Category: Hydrolase]]
-[[Category: monomer]]
+[[Category: Monomer]]
-[[Category: nucleotide metabolism]]
+[[Category: Nucleotide metabolism]]
-[[Category: spine]]
+[[Category: Spine]]
-[[Category: structural genomic]]
+[[Category: Structural genomic]]
-[[Category: structural proteomics in europe]]
+[[Category: Structural proteomics in europe]]
+''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Apr 13 08:18:29 2008''
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:11:20 2008''