114d: Difference between revisions
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==INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX== | ==INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX== | ||
<StructureSection load='114d' size='340' side='right' caption='[[114d]], [[Resolution|resolution]] 2.50Å' scene=''> | <StructureSection load='114d' size='340' side='right'caption='[[114d]], [[Resolution|resolution]] 2.50Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[114d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=114D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=114D FirstGlance]. <br> | <table><tr><td colspan='2'>[[114d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=114D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=114D FirstGlance]. <br> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Brown, T]] | [[Category: Brown, T]] | ||
[[Category: Corfield, P W.R]] | [[Category: Corfield, P W.R]] | ||
Revision as of 17:32, 10 May 2019
INOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEXINOSINE-ADENINE BASE PAIRS IN A B-DNA DUPLEX
Structural highlights
Publication Abstract from PubMedThe structure of the synthetic deoxydodecamer d(C-G-C-I-A-A-T-T-A-G-C-G) has been determined by single crystal X-ray diffraction techniques at 2.5A resolution. The refinement converged with a crystallographic residual, R = 0.19 and the location of 64 solvent molecules. The sequence crystallises as a B-DNA helix with 10 Watson-Crick base-pairs (4 A.T. and 6 G.C) and 2 inosine.adenine (I.A) pairs. The present work shows that in the purine.purine base-pairs the adenine adopts syn orientation with respect to the furanose moiety while the inosine is in the trans (anti) orientation. Two hydrogen bonds link the I.A. base-pair, one between N-1(I) and N-7(A), the other between O-6(I) and N-6(A). This bulky purine.purine base-pair is incorporated in the double helix at two positions with little distortion of either local or global conformation. The pairing observed in this study is presented as a model for I.A base-pairs in RNA codon-anticodon interactions and may help explain the thermodynamic stability of inosine containing base-pairs. Conformational parameters and base stacking interactions are presented and where appropriate compared with those of the native compound, d(C-G-C-G-A-A-T-T-C-G-C-G) and with other studies of oligonucleotides containing purine.purine base-pairs. Inosine.adenine base pairs in a B-DNA duplex.,Corfield PW, Hunter WN, Brown T, Robinson P, Kennard O Nucleic Acids Res. 1987 Oct 12;15(19):7935-49. PMID:3671069[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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