4rvh: Difference between revisions
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==Crystal structure of MtmC in complex with SAH and TDP-4-keto-D-olivose== | ==Crystal structure of MtmC in complex with SAH and TDP-4-keto-D-olivose== | ||
<StructureSection load='4rvh' size='340' side='right' caption='[[4rvh]], [[Resolution|resolution]] 2.40Å' scene=''> | <StructureSection load='4rvh' size='340' side='right'caption='[[4rvh]], [[Resolution|resolution]] 2.40Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4rvh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RVH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RVH FirstGlance]. <br> | <table><tr><td colspan='2'>[[4rvh]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"streptomyces_argillaceus"_routien "streptomyces argillaceus" routien]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RVH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RVH FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3YN:[(2R,3S,5R)-3-HYDROXY-5-(5-METHYL-2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)TETRAHYDROFURAN-2-YL]METHYL+(2R,4R,6R)-4-HYDROXY-6-METHYL-5-OXOTETRAHYDRO-2H-PYRAN-2-YL+DIHYDROGEN+DIPHOSPHATE'>3YN</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=3YN:[(2R,3S,5R)-3-HYDROXY-5-(5-METHYL-2,4-DIOXO-3,4-DIHYDROPYRIMIDIN-1(2H)-YL)TETRAHYDROFURAN-2-YL]METHYL+(2R,4R,6R)-4-HYDROXY-6-METHYL-5-OXOTETRAHYDRO-2H-PYRAN-2-YL+DIHYDROGEN+DIPHOSPHATE'>3YN</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rv9|1rv9]], [[4rvd|4rvd]], [[4rvf|4rvf]], [[4rvg|4rvg]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1rv9|1rv9]], [[4rvd|4rvd]], [[4rvf|4rvf]], [[4rvg|4rvg]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mtmC ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=41951 "Streptomyces argillaceus" Routien])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rvh OCA], [http://pdbe.org/4rvh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rvh RCSB], [http://www.ebi.ac.uk/pdbsum/4rvh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rvh ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rvh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rvh OCA], [http://pdbe.org/4rvh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rvh RCSB], [http://www.ebi.ac.uk/pdbsum/4rvh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rvh ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Streptomyces argillaceus routien]] | |||
[[Category: Large Structures]] | |||
[[Category: Chen, J M]] | [[Category: Chen, J M]] | ||
[[Category: Hou, C]] | [[Category: Hou, C]] | ||
Revision as of 13:23, 1 May 2019
Crystal structure of MtmC in complex with SAH and TDP-4-keto-D-olivoseCrystal structure of MtmC in complex with SAH and TDP-4-keto-D-olivose
Structural highlights
Publication Abstract from PubMedMore and more post-PKS tailoring enzymes are recognized to be multifunctional and co-dependent on other tailoring enzymes. One of the recently discovered intriguing examples is MtmC, a bifunctional TDP-4-keto-D-olivose ketoreductase-methyltransferase, which - in co-dependence with glycosyltransferase MtmGIV - is a key contributor to the biosynthesis of the critical trisaccharide chain of the antitumor antibiotic mithramycin (MTM), produced by Streptomyces argillaceus. We report crystal structures of three binary complexes of MtmC with its methylation co-substrate SAM, its co-product SAH, and a nucleotide TDP as well as crystal structures of two ternary complexes, MtmC-SAH-TDP-4-keto-D-olivose and MtmC-SAM-TDP, in the range of 2.2-2.7 A in resolution. The structures reveal general and sugar-specific recognition and catalytic structural features of MtmC. Depending on the catalytic function that is carried out by MtmC, it must bind either NADPH or SAM in the same co-factor binding pocket. A tyrosine residue (Tyr79) appears as a lid covering the sugar moiety of the substrate during the methyl transfer reaction. This residue swings out of the active site by about 180o in the absence of the substrate. This unique conformational change likely serves to release the methylated product and, possibly, to open up the active site for binding the bulkier co-substrate NADPH prior to the reduction reaction. Structural insight into MtmC, a bifunctional ketoreductase-methyltransferase involved in the assembly of the mithramycin trisaccharide chain.,Chen JM, Hou C, Wang G, Tsodikov OV, Rohr J Biochemistry. 2015 Jan 14. PMID:25587924[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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