4pn6: Difference between revisions
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==Structure of the Cytomegalovirus-Encoded m04 Glycoprotein== | ==Structure of the Cytomegalovirus-Encoded m04 Glycoprotein== | ||
<StructureSection load='4pn6' size='340' side='right' caption='[[4pn6]], [[Resolution|resolution]] 3.00Å' scene=''> | <StructureSection load='4pn6' size='340' side='right'caption='[[4pn6]], [[Resolution|resolution]] 3.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4pn6]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PN6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PN6 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4pn6]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Muromegalovirus_g4 Muromegalovirus g4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PN6 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4PN6 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand= | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">m04 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=524650 Muromegalovirus G4])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pn6 OCA], [http://pdbe.org/4pn6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pn6 RCSB], [http://www.ebi.ac.uk/pdbsum/4pn6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pn6 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4pn6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pn6 OCA], [http://pdbe.org/4pn6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4pn6 RCSB], [http://www.ebi.ac.uk/pdbsum/4pn6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4pn6 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Muromegalovirus g4]] | |||
[[Category: Berry, R]] | [[Category: Berry, R]] | ||
[[Category: Rossjohn, J]] | [[Category: Rossjohn, J]] | ||
[[Category: Viral protein]] | [[Category: Viral protein]] | ||
Revision as of 10:22, 17 April 2019
Structure of the Cytomegalovirus-Encoded m04 GlycoproteinStructure of the Cytomegalovirus-Encoded m04 Glycoprotein
Structural highlights
Publication Abstract from PubMedThe ability of cytomegaloviruses (CMV) to evade the host's immune system is dependent on the expression of a wide array of glycoproteins, many of which interfere with natural killer (NK) cell function. In murine CMV, two large protein families mediate this immune-evasive function. While it is established that the m145 family members mimic the structure of major histocompatibility complex (MHC)-I molecules, the structure of the m02 family remains unknown. The most extensively studied m02 family member is m04, a glycoprotein that escorts newly assembled MHC-I molecules to the cell surface, presumably to avoid missing-self recognition. Here we report the crystal structure of the m04 ectodomain, thereby providing insight into this large immunoevasin family. m04 adopted a beta-sandwich immunoglobulin variable (Ig-V) like fold, despite sharing very little sequence identity with the Ig-V superfamily. In addition to the Ig-V core, m04 possesses several unique structural features that included an unusual beta-strand topology, a number of extended loops and a prominent alpha-helix. The m04 interior was packed by a myriad of hydrophobic residues that form distinct clusters around two conserved tryptophan residues. This hydrophobic core was well conserved throughout the m02 family, thereby indicating that MCMV encodes a number of Ig-V like molecules. We show that m04 binds a range of MHC-I molecules with low affinity in a peptide-independent manner. Accordingly the structure of m04, which represents the first example of an MCMV encoded Ig-V fold, provides a basis for understanding the structure and function of this enigmatic and large family of immunoevasins. The Structure of the Cytomegalovirus-Encoded m04 Glycoprotein, a Prototypical Member of the m02 Family of Immunoevasins.,Berry R, Vivian JP, Deuss FA, Balaji GR, Saunders PM, Lin J, Littler DR, Brooks AG, Rossjohn J J Biol Chem. 2014 Jun 30. pii: jbc.M114.584128. PMID:24982419[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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