6ddu: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==mouse beta-mannosidase bound to beta-D-mannose (MANBA)== | ==mouse beta-mannosidase bound to beta-D-mannose (MANBA)== | ||
<StructureSection load='6ddu' size='340' side='right' caption='[[6ddu]], [[Resolution|resolution]] 2.67Å' scene=''> | <StructureSection load='6ddu' size='340' side='right'caption='[[6ddu]], [[Resolution|resolution]] 2.67Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6ddu]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DDU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DDU FirstGlance]. <br> | <table><tr><td colspan='2'>[[6ddu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DDU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DDU FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Manba, Bmn ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | |||
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-mannosidase Beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.25 3.2.1.25] </span></td></tr> | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Beta-mannosidase Beta-mannosidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.25 3.2.1.25] </span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ddu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ddu OCA], [http://pdbe.org/6ddu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ddu RCSB], [http://www.ebi.ac.uk/pdbsum/6ddu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ddu ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ddu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ddu OCA], [http://pdbe.org/6ddu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ddu RCSB], [http://www.ebi.ac.uk/pdbsum/6ddu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ddu ProSAT]</span></td></tr> | ||
| Line 24: | Line 25: | ||
</StructureSection> | </StructureSection> | ||
[[Category: Beta-mannosidase]] | [[Category: Beta-mannosidase]] | ||
[[Category: Large Structures]] | |||
[[Category: Lk3 transgenic mice]] | |||
[[Category: Gorelik, A]] | [[Category: Gorelik, A]] | ||
[[Category: Gytz, H]] | [[Category: Gytz, H]] | ||
Revision as of 09:44, 17 April 2019
mouse beta-mannosidase bound to beta-D-mannose (MANBA)mouse beta-mannosidase bound to beta-D-mannose (MANBA)
Structural highlights
Function[MANBA_MOUSE] Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.[UniProtKB:O00462] Publication Abstract from PubMedbeta-Mannosidase is a lysosomal enzyme from the glycosyl hydrolase family 2 that cleaves the single beta(1-4)-linked mannose at the nonreducing end of N-glycosylated proteins, and plays an important role in the polysaccharide degradation pathway. Mutations in the MANBA gene, which encodes the beta-mannosidase, can lead to the lysosomal storage disease beta-mannosidosis, as well as nystagmus, an eye condition characterized by involuntary eye movements. Here, we present the first structures of a mammalian beta-mannosidase in both the apo- and mannose-bound forms. The structure is similar to previously determined beta-mannosidase structures with regard to domain organization and fold, however, there are important differences that underlie substrate specificity between species. Additionally, in contrast to most other ligand-bound beta-mannosidases from bacterial and fungal sources where bound sugars were in a boat-like conformation, we find the mannose in the chair conformation. Evaluation of known disease mutations in the MANBA gene provides insight into their impact on disease phenotypes. Together, these results will be important for the design of therapeutics for treating diseases caused by beta-mannosidase deficiency. DATABASE: Structural data are available in the Protein Data Bank under the accession numbers 6DDT and 6DDU. The structure of mammalian beta-mannosidase provides insight into beta-mannosidosis and nystagmus.,Gytz H, Liang J, Liang Y, Gorelik A, Illes K, Nagar B FEBS J. 2018 Dec 15. doi: 10.1111/febs.14731. PMID:30552791[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||||