5cbl: Difference between revisions
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==Crystal structure of the C-terminal domain of human galectin-4 with lactose== | ==Crystal structure of the C-terminal domain of human galectin-4 with lactose== | ||
<StructureSection load='5cbl' size='340' side='right' caption='[[5cbl]], [[Resolution|resolution]] 1.78Å' scene=''> | <StructureSection load='5cbl' size='340' side='right'caption='[[5cbl]], [[Resolution|resolution]] 1.78Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5cbl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CBL FirstGlance]. <br> | <table><tr><td colspan='2'>[[5cbl]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5CBL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5CBL FirstGlance]. <br> | ||
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</div> | </div> | ||
<div class="pdbe-citations 5cbl" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5cbl" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Galectin|Galectin]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Human]] | [[Category: Human]] | ||
[[Category: Large Structures]] | |||
[[Category: Nonato, M C]] | [[Category: Nonato, M C]] | ||
[[Category: Rustiguel, J K]] | [[Category: Rustiguel, J K]] | ||
Revision as of 09:19, 17 April 2019
Crystal structure of the C-terminal domain of human galectin-4 with lactoseCrystal structure of the C-terminal domain of human galectin-4 with lactose
Structural highlights
Function[LEG4_HUMAN] Galectin that binds lactose and a related range of sugars. May be involved in the assembly of adherens junctions. Publication Abstract from PubMedGalectin-4 (Gal4), a tandem-repeat type galectin, is expressed in healthy epithelium of the gastrointestinal tract. Altered levels of Gal4 expression are associated with different types of cancer, suggesting its usage as a diagnostic marker as well as target for drug development. The functional data available for this class of proteins suggest that the wide spectrum of cellular activities reported for Gal4 relies on distinct glycan specificity and structural characteristics of its two carbohydrate recognition domains. In the present work, two independent constructs for recombinant expression of the C-terminal domain of human galectin-4 (hGal4-CRD2) were developed. His6-tagged and untagged recombinant proteins were overexpressed in Escherichia coli, and purified by affinity chromatography followed by gel filtration. Correct folding and activity of hGal4-CRD2 were assessed by circular dichroism and fluorescence spectroscopies, respectively. Diffraction quality crystals were obtained by vapor-diffusion sitting drop setup and the crystal structure of CRD2 was solved by molecular replacement techniques at 1.78 A resolution. Our work describes the development of important experimental tools that will allow further studies in order to correlate structure and binding properties of hGal4-CRD2 and human galectin-4 functional activities. Recombinant expression, purification and preliminary biophysical and structural studies of C-terminal carbohydrate recognition domain from human galectin-4.,Rustiguel JK, Kumagai PS, Dias-Baruffi M, Costa-Filho AJ, Nonato MC Protein Expr Purif. 2015 Oct 24;118:39-48. doi: 10.1016/j.pep.2015.09.026. PMID:26432949[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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