6n0a: Difference between revisions
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==Structure of the major pilin protein (T-18.1) from Streptococcus pyogenes serotype MGAS8232== | |||
<StructureSection load='6n0a' size='340' side='right'caption='[[6n0a]], [[Resolution|resolution]] 1.75Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6n0a]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6N0A OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6N0A FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6n0a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6n0a OCA], [http://pdbe.org/6n0a PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6n0a RCSB], [http://www.ebi.ac.uk/pdbsum/6n0a PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6n0a ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Group A Streptococcus (GAS, Streptococcus pyogenes) is an important human pathogen associated with significant global morbidity and mortality for which there is no safe and efficacious vaccine. The T-antigen, a protein that polymerises to form the backbone of the GAS pilus structure, is a potential vaccine candidate. Previous surveys of the tee gene, which encodes the T-antigen, have identified 21 different tee-types and subtypes such that any T-antigen-based vaccine must be multivalent and carefully designed to provide broad strain coverage. In this study, the crystal structures of three two-domain T-antigens (T3.2, T13 and T18.1) were determined and found to have remarkable structural similarity to the previously reported T1, despite moderate overall sequence similarity. This has enabled reliable modelling of all major two-domain T-antigens to reveal that T-antigen sequence variation is distributed along the full length of the protein, and shields a highly conserved core. Immunoassays performed with sera from immunized animals and commercial T-typing sera identified a significant cross-reactive antibody response between T18.1, T18.2, T3.2 and T13. The existence of shared epitopes between T-antigens, combined with the remarkably conserved structure and high surface sequence divergence, has important implications for the design of multivalent T-antigen-based vaccines. | |||
Group A Streptococcus T-antigens have a highly conserved structure concealed under a heterogeneous surface that has implications for vaccine design.,Young PG, Raynes JM, Loh JM, Proft T, Baker EN, Moreland NJ Infect Immun. 2019 Apr 1. pii: IAI.00205-19. doi: 10.1128/IAI.00205-19. PMID:30936156<ref>PMID:30936156</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6n0a" style="background-color:#fffaf0;"></div> | ||
[[Category: | == References == | ||
[[Category: | <references/> | ||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Baker, E N]] | |||
[[Category: Loh, J M]] | |||
[[Category: Moreland, N J]] | |||
[[Category: Proft, T]] | [[Category: Proft, T]] | ||
[[Category: | [[Category: Raynes, J M]] | ||
[[Category: Young, P | [[Category: Young, P G]] | ||
[[Category: Major pilin backbone protein]] | |||
[[Category: Structural protein]] | |||
[[Category: T-antigen]] | |||
[[Category: T18 1]] | |||
Revision as of 09:02, 17 April 2019
Structure of the major pilin protein (T-18.1) from Streptococcus pyogenes serotype MGAS8232Structure of the major pilin protein (T-18.1) from Streptococcus pyogenes serotype MGAS8232
Structural highlights
Publication Abstract from PubMedGroup A Streptococcus (GAS, Streptococcus pyogenes) is an important human pathogen associated with significant global morbidity and mortality for which there is no safe and efficacious vaccine. The T-antigen, a protein that polymerises to form the backbone of the GAS pilus structure, is a potential vaccine candidate. Previous surveys of the tee gene, which encodes the T-antigen, have identified 21 different tee-types and subtypes such that any T-antigen-based vaccine must be multivalent and carefully designed to provide broad strain coverage. In this study, the crystal structures of three two-domain T-antigens (T3.2, T13 and T18.1) were determined and found to have remarkable structural similarity to the previously reported T1, despite moderate overall sequence similarity. This has enabled reliable modelling of all major two-domain T-antigens to reveal that T-antigen sequence variation is distributed along the full length of the protein, and shields a highly conserved core. Immunoassays performed with sera from immunized animals and commercial T-typing sera identified a significant cross-reactive antibody response between T18.1, T18.2, T3.2 and T13. The existence of shared epitopes between T-antigens, combined with the remarkably conserved structure and high surface sequence divergence, has important implications for the design of multivalent T-antigen-based vaccines. Group A Streptococcus T-antigens have a highly conserved structure concealed under a heterogeneous surface that has implications for vaccine design.,Young PG, Raynes JM, Loh JM, Proft T, Baker EN, Moreland NJ Infect Immun. 2019 Apr 1. pii: IAI.00205-19. doi: 10.1128/IAI.00205-19. PMID:30936156[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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