Bromelain: Difference between revisions
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==Temperature stability== | ==Temperature stability== | ||
At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles. | At the optimum temperature, the enzyme acts the fastest, but (at least the fruit variant) is destroyed within few minutes. After an hour at 50 °C (122 °F), 83% of the enzyme remains, while at 40 °C (104 °F), practically 100% remains. As a result of this, the optimum temperature for maximum cumulated activity over time is 35-45 °C. At room temperature, the enzyme can survive at least a week even under multiple freeze-thaw cycles. | ||
==How it works== | |||
Bromelain is a cysteine protease. The <scene name='60/609840/Active_site/1'>active site</scene> contains a cysteine residue. A neighboring histidine increases the ionization of the cysteine, making it a better nucleophile. | |||
== 3D Structures of bromelain == | == 3D Structures of bromelain == | ||