6gav: Difference between revisions
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==Extremely 'open' clamp structure of DNA gyrase: role of the Corynebacteriales GyrB specific insert== | ==Extremely 'open' clamp structure of DNA gyrase: role of the Corynebacteriales GyrB specific insert== | ||
<StructureSection load='6gav' size='340' side='right' caption='[[6gav]], [[Resolution|resolution]] 2.60Å' scene=''> | <StructureSection load='6gav' size='340' side='right'caption='[[6gav]], [[Resolution|resolution]] 2.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6gav]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAV FirstGlance]. <br> | <table><tr><td colspan='2'>[[6gav]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_tuberculosis"_(zopf_1883)_klein_1884 "bacillus tuberculosis" (zopf 1883) klein 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6GAV OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6GAV FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">gyrA, Rv0006, MTCY10H4.04 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1773 "Bacillus tuberculosis" (Zopf 1883) Klein 1884])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gav OCA], [http://pdbe.org/6gav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gav RCSB], [http://www.ebi.ac.uk/pdbsum/6gav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gav ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6gav FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6gav OCA], [http://pdbe.org/6gav PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6gav RCSB], [http://www.ebi.ac.uk/pdbsum/6gav PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6gav ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Alzari, P M]] | [[Category: Alzari, P M]] | ||
[[Category: Aubry, A]] | [[Category: Aubry, A]] | ||
Latest revision as of 10:48, 10 April 2019
Extremely 'open' clamp structure of DNA gyrase: role of the Corynebacteriales GyrB specific insertExtremely 'open' clamp structure of DNA gyrase: role of the Corynebacteriales GyrB specific insert
Structural highlights
Function[F6N7X0_MYCTX] A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.[HAMAP-Rule:MF_01898] Publication Abstract from PubMedDespite sharing common features, previous studies have shown that gyrases from different species have been modified throughout evolution to modulate their properties. Here, we report two crystal structures of Mycobacterium tuberculosis DNA gyrase, an apo and AMPPNP-bound form at 2.6-A and 3.3-A resolution, respectively. These structures provide high-resolution structural data on the quaternary organization and interdomain connections of a gyrase (full-length GyrB-GyrA57)2 thus providing crucial inputs on this essential drug target. Together with small-angle X-ray scattering studies, they revealed an "extremely open" N-gate state, which persists even in the DNA-free gyrase-AMPPNP complex and an unexpected connection between the ATPase and cleavage core domains mediated by two Corynebacteriales-specific motifs, respectively the C-loop and DEEE-loop. We show that the C-loop participates in the stabilization of this open conformation, explaining why this gyrase has a lower ATPase activity. Our results image a conformational state which might be targeted for drug discovery. Overall Structures of Mycobacterium tuberculosis DNA Gyrase Reveal the Role of a Corynebacteriales GyrB-Specific Insert in ATPase Activity.,Petrella S, Capton E, Raynal B, Giffard C, Thureau A, Bonnete F, Alzari PM, Aubry A, Mayer C Structure. 2019 Feb 7. pii: S0969-2126(19)30004-8. doi:, 10.1016/j.str.2019.01.004. PMID:30744994[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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