6if9: Difference between revisions
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The entry | ==Solution-state NMR structure of G57W human gammaS crystallin== | ||
<StructureSection load='6if9' size='340' side='right'caption='[[6if9]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6if9]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6IF9 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6IF9 FirstGlance]. <br> | |||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6if9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6if9 OCA], [http://pdbe.org/6if9 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6if9 RCSB], [http://www.ebi.ac.uk/pdbsum/6if9 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6if9 ProSAT]</span></td></tr> | |||
</table> | |||
== Disease == | |||
[[http://www.uniprot.org/uniprot/CRYGS_HUMAN CRYGS_HUMAN]] Early-onset lamellar cataract;Early-onset sutural cataract. The disease is caused by mutations affecting the gene represented in this entry. | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/CRYGS_HUMAN CRYGS_HUMAN]] Crystallins are the dominant structural components of the vertebrate eye lens. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
A recently identified mutant of human gammaS-crystallin, G57W is associated with dominant congenital cataracts, the familial determinate of childhood blindness worldwide. To investigate the structural and functional changes that mediate the effect of this cataract-related mutant to compromise eye lens transparency and cause lens opacification in children, we recently reported complete sequence-specific resonance assignments of gammaS-G57W using a suite of heteronuclear NMR experiments. As a follow up, we have determined the 3D structure of gammaS-G57W and studied its conformational dynamics by solution NMR spectroscopy. Our structural dynamics results reveal greater flexibility of the N-terminal domain, which undergoes site-specific structural changes to accommodate W57, than its C-terminal counterpart. Our structural inferences that the unusual solvent exposure of W57 is associated with rearrangement of the N-terminal domain suggest an efficient pathway for increased aggregation in gammaS-G57W and illuminates the molecular dynamics underlying cataractogenic aggregation of lens crystallins in particular and aggregation of proteins in general. | |||
Structure of G57W mutant of human gammaS-crystallin and its involvement in cataract formation.,Bari KJ, Sharma S, Chary KVR J Struct Biol. 2019 Mar 1;205(3):72-78. doi: 10.1016/j.jsb.2019.02.003. Epub 2019, Feb 12. PMID:30769148<ref>PMID:30769148</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6if9" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Large Structures]] | |||
[[Category: Bari, K J]] | |||
[[Category: Chary, K V.R]] | |||
[[Category: Sharma, S]] | [[Category: Sharma, S]] | ||
[[Category: | [[Category: Recombination]] | ||
[[Category: | [[Category: Structure from cyana 3 97]] | ||
Revision as of 10:22, 10 April 2019
Solution-state NMR structure of G57W human gammaS crystallinSolution-state NMR structure of G57W human gammaS crystallin
Structural highlights
Disease[CRYGS_HUMAN] Early-onset lamellar cataract;Early-onset sutural cataract. The disease is caused by mutations affecting the gene represented in this entry. Function[CRYGS_HUMAN] Crystallins are the dominant structural components of the vertebrate eye lens. Publication Abstract from PubMedA recently identified mutant of human gammaS-crystallin, G57W is associated with dominant congenital cataracts, the familial determinate of childhood blindness worldwide. To investigate the structural and functional changes that mediate the effect of this cataract-related mutant to compromise eye lens transparency and cause lens opacification in children, we recently reported complete sequence-specific resonance assignments of gammaS-G57W using a suite of heteronuclear NMR experiments. As a follow up, we have determined the 3D structure of gammaS-G57W and studied its conformational dynamics by solution NMR spectroscopy. Our structural dynamics results reveal greater flexibility of the N-terminal domain, which undergoes site-specific structural changes to accommodate W57, than its C-terminal counterpart. Our structural inferences that the unusual solvent exposure of W57 is associated with rearrangement of the N-terminal domain suggest an efficient pathway for increased aggregation in gammaS-G57W and illuminates the molecular dynamics underlying cataractogenic aggregation of lens crystallins in particular and aggregation of proteins in general. Structure of G57W mutant of human gammaS-crystallin and its involvement in cataract formation.,Bari KJ, Sharma S, Chary KVR J Struct Biol. 2019 Mar 1;205(3):72-78. doi: 10.1016/j.jsb.2019.02.003. Epub 2019, Feb 12. PMID:30769148[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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