4kf4: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal Structure of sfCherry== | ==Crystal Structure of sfCherry== | ||
<StructureSection load='4kf4' size='340' side='right' caption='[[4kf4]], [[Resolution|resolution]] 1.99Å' scene=''> | <StructureSection load='4kf4' size='340' side='right'caption='[[4kf4]], [[Resolution|resolution]] 1.99Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4kf4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KF4 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4kf4]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KF4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KF4 FirstGlance]. <br> | ||
| Line 21: | Line 21: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Synthetic construct sequences]] | [[Category: Synthetic construct sequences]] | ||
[[Category: Hung, L W]] | [[Category: Hung, L W]] | ||
Revision as of 12:07, 3 April 2019
Crystal Structure of sfCherryCrystal Structure of sfCherry
Structural highlights
Publication Abstract from PubMedA modular strategy for protein crystallization using split green fluorescent protein (GFP) as a crystallization partner is demonstrated. Insertion of a hairpin containing GFP beta-strands 10 and 11 into a surface loop of a target protein provides two chain crossings between the target and the reconstituted GFP compared with the single connection afforded by terminal GFP fusions. This strategy was tested by inserting this hairpin into a loop of another fluorescent protein, sfCherry. The crystal structure of the sfCherry-GFP(10-11) hairpin in complex with GFP(1-9) was determined at a resolution of 2.6 A. Analysis of the complex shows that the reconstituted GFP is attached to the target protein (sfCherry) in a structurally ordered way. This work opens the way to rapidly creating crystallization variants by reconstituting a target protein bearing the GFP(10-11) hairpin with a variety of GFP(1-9) mutants engineered for favorable crystallization. Split green fluorescent protein as a modular binding partner for protein crystallization.,Nguyen HB, Hung LW, Yeates TO, Terwilliger TC, Waldo GS Acta Crystallogr D Biol Crystallogr. 2013 Dec;69(Pt 12):2513-23. doi:, 10.1107/S0907444913024608. Epub 2013 Nov 19. PMID:24311592[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||