2c4b: Difference between revisions
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==Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A== | ==Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A== | ||
<StructureSection load='2c4b' size='340' side='right' caption='[[2c4b]], [[Resolution|resolution]] 1.30Å' scene=''> | <StructureSection load='2c4b' size='340' side='right'caption='[[2c4b]], [[Resolution|resolution]] 1.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2c4b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C4B FirstGlance]. <br> | <table><tr><td colspan='2'>[[2c4b]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_amyloliquifaciens"_(sic)_fukumoto_1943 "bacillus amyloliquifaciens" (sic) fukumoto 1943]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2C4B OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2C4B FirstGlance]. <br> | ||
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==See Also== | ==See Also== | ||
*[[Barnase|Barnase]] | *[[Barnase 3D structures|Barnase 3D structures]] | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | |||
[[Category: Heinz, D W]] | [[Category: Heinz, D W]] | ||
[[Category: Kolmar, H]] | [[Category: Kolmar, H]] | ||
Revision as of 10:22, 3 April 2019
Inhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102AInhibitor cystine knot protein McoEeTI fused to the catalytically inactive barnase mutant H102A
Structural highlights
Function[RNBR_BACAM] Hydrolyzes phosphodiester bonds in RNA, poly- and oligoribonucleotides resulting in 3'-nucleoside monophosphates via 2',3'-cyclophosphate intermediates. Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedWe present a fusion system suited to determine the crystal structure of small disulfide-rich proteins. McoEeTI, a hybrid inhibitor cystine knot microprotein, was produced as a soluble fusion to a catalytically inactive variant of the RNAse barnase in Escherichia coli. Functioning as a versatile tag, barnase facilitated purification, crystallization and high-resolution structure determination. Flexibility of the linker region allows for different relative orientations of barnase and the fusion partner in two crystallographically independent molecules and may thereby facilitate crystal packing. Nevertheless, the linker region is well ordered in both molecules. This system may prove more generally useful to determine the crystal structure of peptides and small proteins. Barnase fusion as a tool to determine the crystal structure of the small disulfide-rich protein McoEeTI.,Niemann HH, Schmoldt HU, Wentzel A, Kolmar H, Heinz DW J Mol Biol. 2006 Feb 10;356(1):1-8. Epub 2005 Nov 21. PMID:16337652[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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