4e3r: Difference between revisions
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==PLP-bound aminotransferase mutant crystal structure from Vibrio fluvialis== | ==PLP-bound aminotransferase mutant crystal structure from Vibrio fluvialis== | ||
<StructureSection load='4e3r' size='340' side='right' caption='[[4e3r]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='4e3r' size='340' side='right'caption='[[4e3r]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4e3r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33809 Atcc 33809]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E3R FirstGlance]. <br> | <table><tr><td colspan='2'>[[4e3r]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_33809 Atcc 33809]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4E3R OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4E3R FirstGlance]. <br> | ||
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</StructureSection> | </StructureSection> | ||
[[Category: Atcc 33809]] | [[Category: Atcc 33809]] | ||
[[Category: Large Structures]] | |||
[[Category: Anderson, M]] | [[Category: Anderson, M]] | ||
[[Category: Chang, J S]] | [[Category: Chang, J S]] | ||
Revision as of 10:43, 27 March 2019
PLP-bound aminotransferase mutant crystal structure from Vibrio fluvialisPLP-bound aminotransferase mutant crystal structure from Vibrio fluvialis
Structural highlights
Publication Abstract from PubMedSeveral protein engineering approaches were combined to optimize the selectivity and activity of Vibrio fluvialis aminotransferase (Vfat) for the synthesis of (3S,5R)-ethyl 3-amino-5-methyloctanoate; a key intermediate in the synthesis of imagabalin, an advanced candidate for the treatment of generalized anxiety disorder. Starting from wild-type Vfat, which had extremely low activity catalyzing the desired reaction, we engineered an improved enzyme with a 60-fold increase in initial reaction velocity for transamination of (R)-ethyl 5-methyl 3-oxooctanoate to (3S,5R)-ethyl 3-amino-5-methyloctanoate. To achieve this, <450 variants were screened, which allowed accurate assessment of enzyme performance using a low-throughput ultra performance liquid chromatography assay. During the course of this work, crystal structures of Vfat wild type and an improved variant (Vfat variant r414) were solved and they are reported here for the first time. This work also provides insight into the critical residues for substrate specificity for the transamination of (R)-ethyl 5-methyl 3-oxooctanoate and structurally related beta-ketoesters. Redesigning and characterizing the substrate specificity and activity of Vibrio fluvialis aminotransferase for the synthesis of imagabalin.,Midelfort KS, Kumar R, Han S, Karmilowicz MJ, McConnell K, Gehlhaar DK, Mistry A, Chang JS, Anderson M, Villalobos A, Minshull J, Govindarajan S, Wong JW Protein Eng Des Sel. 2012 Sep 25. PMID:23012440[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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