6ns4: Difference between revisions

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-'''Unreleased structure'''
-The entry 6ns4 is ON HOLD  until Paper Publication
+==Crystal structure of fungal lipoxygenase from Fusarium graminearum. C2 crystal form.==
+<StructureSection load='6ns4' size='340' side='right'caption='[[6ns4]], [[Resolution|resolution]] 2.40&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6ns4]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NS4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6NS4 FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6ns2|6ns2]], [[6ns3|6ns3]], [[6ns5|6ns5]], [[6ns6|6ns6]]</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ns4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ns4 OCA], [http://pdbe.org/6ns4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ns4 RCSB], [http://www.ebi.ac.uk/pdbsum/6ns4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ns4 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The regio- and stereo- specific oxygenation of polyunsaturated fatty acids (PUFA) is catalyzed by lipoxygenases (LOX); both Fe and Mn forms of the enzyme have been described. Structural elements of the Fe and Mn coordination spheres and the helical catalytic domain in which the metal center resides are highly conserved. However, animal, plant and microbial LOX each have distinct features. We report five crystal structures of a LOX from the fungal plant pathogen Fusarium graminearum. This LOX displays a novel amino terminal extension that provides a wrapping domain for dimerization. Moreover, this extension appears to interfere with the iron coordination sphere, as the typical LOX configuration is not observed at the catalytic metal when the enzyme is dimeric. Instead novel tetra-, penta- and hexa- coordinate Fe(2+) ligations are apparent. In contrast, a monomeric structure indicates that with repositioning of the amino terminal segment, the enzyme can assume a productive conformation with the canonical Fe(2+) coordination sphere. This article is protected by copyright. All rights reserved.
-Authors: Pakhomova, S., Boeglin, W.E., Neau, D.B., Bartlett, S.G., Brash, A.R., Newcomer, M.E.
+An ensemble of lipoxygenase structures reveals novel conformations of the Fe coordination sphere.,Pakhomova S, Boeglin WE, Neau DB, Bartlett SG, Brash AR, Newcomer ME Protein Sci. 2019 Mar 12. doi: 10.1002/pro.3602. PMID:30861228<ref>PMID:30861228</ref>
-Description: Crystal structure of fungal lipoxygenase from Fusarium graminearum. C2 crystal form.
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Brash, A.R]]
+<div class="pdbe-citations 6ns4" style="background-color:#fffaf0;"></div>
-[[Category: Neau, D.B]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Bartlett, S G]]
+[[Category: Boeglin, W E]]
+[[Category: Brash, A R]]
+[[Category: Neau, D B]]
+[[Category: Newcomer, M E]]
 [[Category: Pakhomova, S]]
-[[Category: Newcomer, M.E]]
+[[Category: Fe coordination]]
-[[Category: Bartlett, S.G]]
+[[Category: Fungus]]
-[[Category: Boeglin, W.E]]
+[[Category: Lipoxygenase]]
+[[Category: Oxidoreductase]]