2cjb: Difference between revisions

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CRYSTAL STRUCTURE OF METHANOSARCINA BARKERI SERYL-TRNA SYNTHETASE COMPLEXED WITH SERINE

OverviewOverview

Methanogenic archaea possess unusual seryl-tRNA synthetase (SerRS), evolutionarily distinct from the SerRSs found in other archaea, eucaryotes, and bacteria. The two types of SerRSs show only minimal sequence, similarity, primarily within class II conserved motifs 1, 2 and 3. Here, we report a 2.5 A resolution crystal structure of the atypical, methanogenic Methanosarcina barkeri SerRS and its complexes with ATP, serine and the nonhydrolysable seryl-adenylate analogue, 5'-O-(N-serylsulfamoyl)adenosine. The structures reveal two idiosyncratic, features of methanogenic SerRSs: a novel N-terminal tRNA-binding domain, and an active site zinc ion. The tetra-coordinated Zn2+ ion is bound to, three conserved protein ligands (Cys306, Glu355 and Cys461) and binds the, amino group of the serine substrate. The absolute requirement of the metal, ion for enzymatic activity was confirmed by mutational analysis of the, direct zinc ion ligands. This zinc-dependent serine recognition mechanism, differs fundamentally from the one employed by the bacterial-type SerRSs., Consequently, SerRS represents the only known aminoacyl-tRNA synthetase, system that evolved two distinct mechanisms for the recognition of the, same amino-acid substrate.

About this StructureAbout this Structure

2CJB is a Single protein structure of sequence from Methanosarcina barkeri with ZN, CL and SER as ligands. Active as Serine--tRNA ligase, with EC number 6.1.1.11 Structure known Active Site: AC1. Full crystallographic information is available from OCA.

ReferenceReference

Structure of the unusual seryl-tRNA synthetase reveals a distinct zinc-dependent mode of substrate recognition., Bilokapic S, Maier T, Ahel D, Gruic-Sovulj I, Soll D, Weygand-Durasevic I, Ban N, EMBO J. 2006 Jun 7;25(11):2498-509. Epub 2006 May 4. PMID:16675947

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	[[Category: zinc ion]]		[[Category: zinc ion]]

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