4cs7: Difference between revisions
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==Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer, form 1== | ==Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer, form 1== | ||
<StructureSection load='4cs7' size='340' side='right' caption='[[4cs7]], [[Resolution|resolution]] 2.47Å' scene=''> | <StructureSection load='4cs7' size='340' side='right'caption='[[4cs7]], [[Resolution|resolution]] 2.47Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4cs7]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS7 FirstGlance]. <br> | <table><tr><td colspan='2'>[[4cs7]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Hmpv Hmpv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4CS7 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4CS7 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cs8|4cs8]], [[4cs9|4cs9]], [[4csa|4csa]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4cs8|4cs8]], [[4cs9|4cs9]], [[4csa|4csa]]</td></tr> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Hmpv]] | |||
[[Category: Large Structures]] | |||
[[Category: Grimes, J M]] | [[Category: Grimes, J M]] | ||
[[Category: Harlos, K]] | [[Category: Harlos, K]] | ||
Revision as of 12:43, 20 March 2019
Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer, form 1Crystal structure of the asymmetric human metapneumovirus M2-1 tetramer, form 1
Structural highlights
Publication Abstract from PubMedThe M2-1 protein of human metapneumovirus (HMPV) is a zinc-binding transcription antiterminator which is highly conserved among pneumoviruses. We report the structure of tetrameric HMPV M2-1. Each protomer features a N-terminal zinc finger domain and an alpha-helical tetramerization motif forming a rigid unit, followed by a flexible linker and an alpha-helical core domain. The tetramer is asymmetric, three of the protomers exhibiting a closed conformation, and one an open conformation. Molecular dynamics simulations and SAXS demonstrate a dynamic equilibrium between open and closed conformations in solution. Structures of adenosine monophosphate- and DNA- bound M2-1 establish the role of the zinc finger domain in base-specific recognition of RNA. Binding to 'gene end' RNA sequences stabilized the closed conformation of M2-1 leading to a drastic shift in the conformational landscape of M2-1. We propose a model for recognition of gene end signals and discuss the implications of these findings for transcriptional regulation in pneumoviruses. Drastic changes in conformational dynamics of the antiterminator M2-1 regulate transcription efficiency in Pneumovirinae.,Leyrat C, Renner M, Harlos K, Huiskonen JT, Grimes JM Elife. 2014 May 19:e02674. doi: 10.7554/eLife.02674. PMID:24842877[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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