6abx: Difference between revisions

← Older edit Newer edit →

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

@@ Line 1: / Line 1: @@
-'''Unreleased structure'''
-The entry 6abx is ON HOLD  until Paper Publication
+==Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with citrate==
+<StructureSection load='6abx' size='340' side='right'caption='[[6abx]], [[Resolution|resolution]] 1.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6abx]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ABX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ABX FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_synthase_(unknown_stereospecificity) Citrate synthase (unknown stereospecificity)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.16 2.3.3.16] </span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6abx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6abx OCA], [http://pdbe.org/6abx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6abx RCSB], [http://www.ebi.ac.uk/pdbsum/6abx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6abx ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Metallosphaera sedula is a thermoacidophilic archaeon and has an incomplete TCA/glyoxylate cycle that is used for production of biosynthetic precursors of essential metabolites. Citrate synthase from M. sedula (MsCS) is an enzyme involved in the first step of the incomplete TCA/glyoxylate cycle by converting oxaloacetate and acetyl-CoA into citrate and coenzyme A. To elucidate the inhibition properties of MsCS, we determined its crystal structure at 1.7 A resolution. Like other Type-I CS, MsCS functions as a dimer and each monomer consists of two distinct domains, a large domain and a small domain. The oxaloacetate binding site locates at the cleft between the two domains, and the active site was more closed upon binding of the oxaloacetate substrate than binding of the citrate product. Interestingly, the inhibition kinetic analysis showed that, unlike other Type-I CSs, MsCS is non-competitively inhibited by NADH. Finally, amino acids and structural comparison of MsCS with other Type-II CSs, which were reported to be non-competitively inhibited by NADH, revealed that MsCS has quite unique NADH binding mode for non-competitive inhibition.
-Authors: Lee, S.-H., Son, H.-F., Kim, K.-J.
+Structural insights into the inhibition properties of archaeon citrate synthase from Metallosphaera sedula.,Lee SH, Son HF, Kim KJ PLoS One. 2019 Feb 22;14(2):e0212807. doi: 10.1371/journal.pone.0212807., eCollection 2019. PMID:30794680<ref>PMID:30794680</ref>
-Description: Crystal structure of citrate synthase (Msed_1522) from Metallosphaera sedula in complex with citrate
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Kim, K.-J]]
+<div class="pdbe-citations 6abx" style="background-color:#fffaf0;"></div>
-[[Category: Lee, S.-H]]
+== References ==
-[[Category: Son, H.-F]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Kim, K J]]
+[[Category: Lee, S H]]
+[[Category: Son, H F]]
+[[Category: Citrate synthase]]
+[[Category: Metallosphaera sedula]]
+[[Category: Transferase]]