Sandbox Reserved 1502: Difference between revisions
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HIV integrase is an enzyme required for the integration of viral DNA into the host genome. The process of integration can be divided into two sequential reactions. In the first one, named 3'-processing, the enzyme removes di- or trinucleotides from viral DNA ends to expose 3′-hydroxyls attached to the invariant CA dinucleotides. The second step is the insertion of the processed 3′-viral DNA ends into the host chromosomal DNA by a trans-esterification reaction. This is the strand transfer. | HIV integrase is an enzyme required for the integration of viral DNA into the host genome. The process of integration can be divided into two sequential reactions. In the first one, named 3'-processing, the enzyme removes di- or trinucleotides from viral DNA ends to expose 3′-hydroxyls attached to the invariant CA dinucleotides. The second step is the insertion of the processed 3′-viral DNA ends into the host chromosomal DNA by a trans-esterification reaction. This is the strand transfer. | ||
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The molecule then inhibits the interaction between the LEDGF/p75 cofactor and the integrase. | The molecule then inhibits the interaction between the LEDGF/p75 cofactor and the integrase. | ||
The whole complex between the integrase and the cofactor is displayed on the Proteopedia page [[2b4j]]. | The whole complex between the integrase and the cofactor is displayed on the Proteopedia page [[2b4j]]. | ||
== Interaction with LEDGF/p75 == | |||
LEDGF/p75, or Lens epithelium-derived growth factor, is a cellular cofactor of HIV integrase. It promotes the viral integration of the retrovirus by tethering the preintegration complex to the chromatin. Research on the interaction between LEDGF/p75 and the integrase shows that it is possible to inhibit this interaction which prevents the viral DNA to be inserted into the genome of the host cell. | |||