Sandbox Reserved 1502: Difference between revisions
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HIV-1 integrase is composed of three domains: the N-terminal (residues 1-49), the core domain (residues 50-212) and the C-terminal domain (residues 213-288). The core domain is responsible for the catalytic activity of the enzyme. It contains three acidic residues, the D,D-35-E motif which plays a key role in catalysis. The N-terminal domain includes the conserved HHCC motif, which binds zinc. The C-terminal domain is less well conserved. [http://www.jbc.org/content/276/26/23213/F2.expansion.html] | HIV-1 integrase is composed of three domains: the N-terminal (residues 1-49), the core domain (residues 50-212) and the C-terminal domain (residues 213-288). The core domain is responsible for the catalytic activity of the enzyme. It contains three acidic residues, the D,D-35-E motif which plays a key role in catalysis. The N-terminal domain includes the conserved HHCC motif, which binds zinc. The C-terminal domain is less well conserved. [http://www.jbc.org/content/276/26/23213/F2.expansion.html] | ||
====Structure and role of the core domain in the integration==== | ====Structure and role of the core domain in the integration==== | ||
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[[Image:HIV Integrase sequence.jpg]] | [[Image:HIV Integrase sequence.jpg]] | ||
====Structure and role of the core domain==== | |||
====Structure and role of the N ter domain==== | |||
The N-terminal domain of HIV-1 integrase contains a conserved pair of His and Cys residues, a motif similar to the zinc-coordinating residues of zinc fingers. This domain binds zinc but its structure is totally different from that of zinc fingers. It consists of a bundle of three α-helices. It has an SH3 fold, although there is no known functional relationship with the SH3 domains of other proteins. | |||
[[Image:Nter-domain.jpg]] | |||
====Structure and interaction with the antiviral==== | ====Structure and interaction with the antiviral==== | ||
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==See also== | ==See also== | ||
http://proteopedia.org/wiki/index.php/2b4j :integrase ligated with the cofactor LEDGF/p75 | http://proteopedia.org/wiki/index.php/2b4j :integrase ligated with the cofactor LEDGF/p75 | ||
== References == | == References == | ||