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==Crystal structure of the medfly Odorant Binding Protein CcapOBP22/CcapOBP69a== | |||
<StructureSection load='6hhe' size='340' side='right' caption='[[6hhe]], [[Resolution|resolution]] 1.52Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6hhe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HHE FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hhe OCA], [http://pdbe.org/6hhe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hhe RCSB], [http://www.ebi.ac.uk/pdbsum/6hhe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hhe ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
In insects, odorant-binding proteins (OBPs) connect the peripheral sensory system to receptors of olfactory organs. Medfly Ceratitis capitata CcapObp22 shows 37% identity and close phylogenetic affinities with Drosophila melanogaster OBP69a/PB-PRP1 (pheromone-binding protein related protein 1). The CcapObp22 gene is transcribed in the antennae and maxillary palps, suggesting an active role in olfaction. Here, we recombinantly produced CcapObp22, obtaining a 13.5 kDa protein capable of binding multiple strongly hydrophobic terpene compounds, including medfly male pheromone components. The highest binding affinity (EC50 0.48 muM) was to (E,E)-alpha-farnesene, one of the most abundant compounds in the male pheromone blend. This odorant was used in co-crystallization experiments, yielding the structure of CcapOBP22. The monomeric structure shows the typical OBP folding, constituted by six alpha-helical elements interconnected by three disulfide bridges. A C-terminal seventh alpha-helix constitutes the wall of a deep, L-shaped hydrophobic cavity. Analysis of the electron density in this cavity suggested trapping of farnesene in the crystal structure, although with partial occupancy. Superposition of the CcapOBP22 structure with related 7-helical OBPs highlights striking similarity in the organization of the C-terminal segment of these proteins. Collectively, our molecular and physiological data on medfly CcapOBP22 suggest its involvement in inter-sex olfactory communication. This article is protected by copyright. All rights reserved. | |||
Structural and biochemical evaluation of Ceratitis capitata OBP22 affinity for odorants involved in inter-sex communication.,Falchetto M, Ciossani G, Scolari F, Di Cosimo A, Nenci S, Field LM, Mattevi A, Zhou JJ, Gasperi G, Forneris F Insect Mol Biol. 2018 Dec 12. doi: 10.1111/imb.12559. PMID:30548711<ref>PMID:30548711</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6hhe" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Ciossani, G]] | [[Category: Ciossani, G]] | ||
[[Category: Falchetto, M]] | [[Category: Falchetto, M]] | ||
[[Category: Forneris, F]] | |||
[[Category: Gasperi, G]] | [[Category: Gasperi, G]] | ||
[[Category: Mattevi, A]] | [[Category: Mattevi, A]] | ||
[[Category: Nenci, S]] | |||
[[Category: Insect olfaction]] | |||
[[Category: Obp]] | |||
[[Category: Odorant-binding protein]] | |||
[[Category: Pheromone]] | |||
[[Category: Transport protein]] | |||