2c49

File:2c49.gif

spinqualitylabelsall models 2c49, resolution 1.92Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF METHANOCALDOCOCCUS JANNASCHII NUCLEOSIDE KINASE- AN ARCHAEAL MEMBER OF THE RIBOKINASE FAMILY

OverviewOverview

Nucleoside kinase from the hyperthermophilic archaeon Methanocaldococcus, jannaschii (MjNK) is a member of the ribokinase family. In the presence of, ATP and Mg(2+), MjNK is able to catalyze the phosphorylation of a variety, of nucleosides, including inosine, cytidine, guanosine and adenosine., Here, the crystal structure of MjNK, the first structure of an archaeal, representative of the ribokinase family, is presented. The structure was, solved using the multiple-wavelength anomalous dispersion technique., Three-dimensional structures of the unliganded enzyme and a complex of, MjNK, an ATP analogue and adenosine were determined to 1.7 and 1.9 A, resolution, respectively. Each subunit comprises an alpha/beta-domain and, a smaller lid domain and has an overall fold characteristic of the, ribokinase superfamily. MjNK shares highest structural similarity to the, ribokinases from Escherichia coli and Thermotoga maritima. Similar to, ribokinase and other superfamily members, the lid domain of MjNK undergoes, a significant conformational change upon substrate binding. In the crystal, structure of the MjNK complex, subunit A adopts a closed conformation and, subunit B an open conformation. In subunit A all substrates and Mg(2+), were observed, whereas in subunit B only the ATP analogue could be clearly, identified in the electron density. The structures of MjNK and E. coli, ribokinase (EcRK) were compared with respect to putative determinants of, thermal stability. Relative to EcRK, MjNK shows an increased charged and a, decreased hydrophobic accessible surface area, as well as a higher, fraction of charged residues, ionic networks and large aromatic clusters, characteristics that are frequently observed in enzymes from, hyperthermophiles.

About this StructureAbout this Structure

2C49 is a Single protein structure of sequence from Methanocaldococcus jannaschii with MG, ADN and ANP as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.

ReferenceReference

Structure of Methanocaldococcus jannaschii nucleoside kinase: an archaeal member of the ribokinase family., Arnfors L, Hansen T, Schonheit P, Ladenstein R, Meining W, Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1085-97. Epub 2006, Aug 19. PMID:16929110

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