3htk: Difference between revisions
No edit summary |
No edit summary |
||
| Line 1: | Line 1: | ||
==Crystal structure of Mms21 and Smc5 complex== | ==Crystal structure of Mms21 and Smc5 complex== | ||
<StructureSection load='3htk' size='340' side='right' caption='[[3htk]], [[Resolution|resolution]] 2.31Å' scene=''> | <StructureSection load='3htk' size='340' side='right' caption='[[3htk]], [[Resolution|resolution]] 2.31Å' scene=''> | ||
| Line 5: | Line 6: | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SMC5, YOL034W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), MMS21, NSE2, YEL019C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SMC5, YOL034W ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824]), MMS21, NSE2, YEL019C ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 ATCC 18824])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3htk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3htk OCA], [http://pdbe.org/3htk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3htk RCSB], [http://www.ebi.ac.uk/pdbsum/3htk PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3htk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3htk OCA], [http://pdbe.org/3htk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3htk RCSB], [http://www.ebi.ac.uk/pdbsum/3htk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3htk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| Line 13: | Line 14: | ||
Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/3htk_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ht/3htk_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
| Line 41: | Line 42: | ||
[[Category: Atp-binding]] | [[Category: Atp-binding]] | ||
[[Category: Chromosomal protein]] | [[Category: Chromosomal protein]] | ||
[[Category: Coiled coil]] | |||
[[Category: Cytoplasm]] | |||
[[Category: Dna damage]] | [[Category: Dna damage]] | ||
[[Category: Dna recombination]] | [[Category: Dna recombination]] | ||
| Line 52: | Line 55: | ||
[[Category: Spl-ring]] | [[Category: Spl-ring]] | ||
[[Category: Sumo e3 ligase]] | [[Category: Sumo e3 ligase]] | ||
[[Category: Ubl conjugation]] | |||
[[Category: Ubl conjugation pathway]] | [[Category: Ubl conjugation pathway]] | ||
[[Category: Zinc]] | |||
[[Category: Zinc-finger]] | [[Category: Zinc-finger]] | ||
Revision as of 11:16, 12 December 2018
Crystal structure of Mms21 and Smc5 complexCrystal structure of Mms21 and Smc5 complex
Structural highlights
Function[SMC5_YEAST] Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks. [NSE2_YEAST] Acts as an E3 ligase mediating SUMO/Smt3 attachment to SMC5 and YKU70. Acts in a DNA repair pathway for removal of UV-induced DNA damage that is distinct from classical nucleotide excision repair and in repair of ionizing radiation damage. Functions in homologous recombination repair of DNA double strand breaks and in recovery of stalled replication forks.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe Smc5/6 complex is an evolutionarily conserved chromosomal ATPase required for cell growth and DNA repair. Its Mms21 subunit supports both functions by docking to the arm region of Smc5 and providing SUMO ligase activity. Here, we report the crystal structure of Mms21 in complex with the Smc5 arm. Our structure revealed two distinct structural and functional domains of the Smc5-bound Mms21: its N-terminal half is dedicated to Smc5 binding by forming a helix bundle with a coiled-coil structure of Smc5; its C-terminal half includes the SUMO ligase domain, which adopts a new type of RING E3 structure. Mutagenesis and structural analyses showed that the Mms21-Smc5 interface is required for cell growth and resistance to DNA damage, while the unique Mms21 RING domain confers specificity to the SUMO E2-E3 interaction. Through structure-based dissection of Mms21 functions, our studies establish a framework for understanding its roles in the Smc5/6 complex. Structural and functional insights into the roles of the Mms21 subunit of the Smc5/6 complex.,Duan X, Sarangi P, Liu X, Rangi GK, Zhao X, Ye H Mol Cell. 2009 Sep 11;35(5):657-68. PMID:19748359[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||
Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Atcc 18824
- Duan, X
- Liu, X
- Rangi, G K
- Sarangi, P
- Ye, H
- Zhao, X
- Atp-binding
- Chromosomal protein
- Coiled coil
- Cytoplasm
- Dna damage
- Dna recombination
- Dna repair
- Ligase
- Metal-binding
- Nucleotide-binding
- Nucleus
- Recombination-replication-ligase complex
- Ring
- Spl-ring
- Sumo e3 ligase
- Ubl conjugation
- Ubl conjugation pathway
- Zinc
- Zinc-finger