Sandbox Reserved 1474: Difference between revisions
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====Radioimmunoassay determination of mAb6H4 cross-reactivity and dissociation constants==== | ====Radioimmunoassay determination of mAb6H4 cross-reactivity and dissociation constants==== | ||
Screening for anti(+)METH IgG response was conducted by radioimmunoassay (RIA)<ref name="Using hapten design to discover therapeutic monoclonal antibodies for treating methamphetamine abuse"/> in which radio labelled [(+)-3H] METH is competing with unlabelled (+)-METH and (+)-AMP for the binding site of Abs in a manner similar to what has been previously described<ref name ="Antibodies against arylcyclohexylamines and their similarities in binding specificity with the phencyclidine receptor"/>. An IC50 value was determined for each compound after fitting a sigmoidal curve to the data points<ref name="Pharmacodynamic mechanisms of monoclonal antibody-based antagonism of (+)-methamphetamine in rats"/><ref name="Using hapten design to discover therapeutic monoclonal antibodies for treating methamphetamine abuse"/>. | Screening for anti(+)METH IgG response was conducted by radioimmunoassay (RIA)<ref name="Using hapten design to discover therapeutic monoclonal antibodies for treating methamphetamine abuse"/> in which radio labelled [(+)-3H] METH is competing with unlabelled (+)-METH and (+)-AMP for the binding site of Abs in a manner similar to what has been previously described<ref name ="Antibodies against arylcyclohexylamines and their similarities in binding specificity with the phencyclidine receptor"/>. An IC50 value was determined for each compound after fitting a sigmoidal curve to the data points<ref name="Pharmacodynamic mechanisms of monoclonal antibody-based antagonism of (+)-methamphetamine in rats"/><ref name="Using hapten design to discover therapeutic monoclonal antibodies for treating methamphetamine abuse"/>. | ||
====Effect of mAb6H4 on METH and AMP pharmacokinetics==== | ====Effect of mAb6H4 on METH and AMP pharmacokinetics==== | ||