2z8d: Difference between revisions
New page: left|200px {{Structure |PDB= 2z8d |SIZE=350|CAPTION= <scene name='initialview01'>2z8d</scene>, resolution 1.85Å |SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+... |
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|PDB= 2z8d |SIZE=350|CAPTION= <scene name='initialview01'>2z8d</scene>, resolution 1.85Å | |PDB= 2z8d |SIZE=350|CAPTION= <scene name='initialview01'>2z8d</scene>, resolution 1.85Å | ||
|SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+For+Residue+A+2'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+1'>AC2</scene>, <scene name='pdbsite=AC3:Nag+Binding+Site+For+Residue+B+2'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+B+1'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+A+3'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+B+3'>AC6</scene>, <scene name='pdbsite=AC7:Zn+Binding+Site+For+Residue+A+4'>AC7</scene>, <scene name='pdbsite=AC8:Zn+Binding+Site+For+Residue+B+4'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Residue+B+5'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Residue+B+6'>BC1</scene>, <scene name='pdbsite=BC2:Zn+Binding+Site+For+Residue+B+7'>BC2</scene>, <scene name='pdbsite=BC3:Mes+Binding+Site+For+Residue+A+11'>BC3</scene> and <scene name='pdbsite=BC4:Mes+Binding+Site+For+Residue+B+12'>BC4</scene> | |SITE= <scene name='pdbsite=AC1:Nag+Binding+Site+For+Residue+A+2'>AC1</scene>, <scene name='pdbsite=AC2:Gal+Binding+Site+For+Residue+A+1'>AC2</scene>, <scene name='pdbsite=AC3:Nag+Binding+Site+For+Residue+B+2'>AC3</scene>, <scene name='pdbsite=AC4:Gal+Binding+Site+For+Residue+B+1'>AC4</scene>, <scene name='pdbsite=AC5:Zn+Binding+Site+For+Residue+A+3'>AC5</scene>, <scene name='pdbsite=AC6:Zn+Binding+Site+For+Residue+B+3'>AC6</scene>, <scene name='pdbsite=AC7:Zn+Binding+Site+For+Residue+A+4'>AC7</scene>, <scene name='pdbsite=AC8:Zn+Binding+Site+For+Residue+B+4'>AC8</scene>, <scene name='pdbsite=AC9:Zn+Binding+Site+For+Residue+B+5'>AC9</scene>, <scene name='pdbsite=BC1:Zn+Binding+Site+For+Residue+B+6'>BC1</scene>, <scene name='pdbsite=BC2:Zn+Binding+Site+For+Residue+B+7'>BC2</scene>, <scene name='pdbsite=BC3:Mes+Binding+Site+For+Residue+A+11'>BC3</scene> and <scene name='pdbsite=BC4:Mes+Binding+Site+For+Residue+B+12'>BC4</scene> | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= gltA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216816 Bifidobacterium longum]) | |GENE= gltA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=216816 Bifidobacterium longum]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2z8e|2Z8E]], [[2z8f|2Z8F]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z8d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z8d OCA], [http://www.ebi.ac.uk/pdbsum/2z8d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z8d RCSB]</span> | |||
}} | }} | ||
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[[Category: Suzuki, R.]] | [[Category: Suzuki, R.]] | ||
[[Category: Wada, J.]] | [[Category: Wada, J.]] | ||
[[Category: abc transporter]] | [[Category: abc transporter]] | ||
[[Category: human milk oligosacchalide]] | [[Category: human milk oligosacchalide]] | ||
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[[Category: sugar binding protein]] | [[Category: sugar binding protein]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:19:22 2008'' | ||
Revision as of 05:19, 31 March 2008
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| , resolution 1.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | , , , , , , , , , , , and | ||||||
| Ligands: | , , , | ||||||
| Gene: | gltA (Bifidobacterium longum) | ||||||
| Related: | 2Z8E, 2Z8F
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum in complex with lacto-N-biose
OverviewOverview
A recombinant galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) from Bifidobacterium longum JCM1217 has been prepared and crystallized by the hanging-drop vapour-diffusion method using 10 mg ml(-1) purified enzyme, 0.01 M zinc sulfate, 0.1 M MES buffer pH 5.9-6.4 and 20-22%(v/v) PEG MME 550 in the presence of 5 mM disaccharide ligands. Suitable crystals grew after 10 d incubation at 293 K. The crystals belong to space group C222(1), with unit-cell parameters a = 106.3, b = 143.6, c = 114.6 A for the lacto-N-biose I complex and a = 106.4, b = 143.4, c = 115.5 A for the galacto-N-biose complex, and diffracted to 1.85 and 1.99 A resolution, respectively.
About this StructureAbout this Structure
2Z8D is a Single protein structure of sequence from Bifidobacterium longum. Full crystallographic information is available from OCA.
ReferenceReference
Purification, crystallization and preliminary X-ray analysis of the galacto-N-biose-/lacto-N-biose I-binding protein (GL-BP) of the ABC transporter from Bifidobacterium longum JCM1217., Wada J, Suzuki R, Fushinobu S, Kitaoka M, Wakagi T, Shoun H, Ashida H, Kumagai H, Katayama T, Yamamoto K, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Sep 1;63(Pt, 9):751-3. Epub 2007 Aug 10. PMID:17768345
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