3f3e: Difference between revisions
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==Crystal structure of LeuT bound to L-leucine (30 mM) and sodium== | ==Crystal structure of LeuT bound to L-leucine (30 mM) and sodium== | ||
<StructureSection load='3f3e' size='340' side='right' caption='[[3f3e]], [[Resolution|resolution]] 1.80Å' scene=''> | <StructureSection load='3f3e' size='340' side='right' caption='[[3f3e]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a65|2a65]], [[2qei|2qei]], [[3f3a|3f3a]], [[3f3c|3f3c]], [[3f3d|3f3d]], [[3f48|3f48]], [[3f4i|3f4i]], [[3f4j|3f4j]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2a65|2a65]], [[2qei|2qei]], [[3f3a|3f3a]], [[3f3c|3f3c]], [[3f3d|3f3d]], [[3f48|3f48]], [[3f4i|3f4i]], [[3f4j|3f4j]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">snf, aq_2077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">snf, aq_2077 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f3e OCA], [http://pdbe.org/3f3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f3e RCSB], [http://www.ebi.ac.uk/pdbsum/3f3e PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3f3e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3f3e OCA], [http://pdbe.org/3f3e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3f3e RCSB], [http://www.ebi.ac.uk/pdbsum/3f3e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3f3e ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/3f3e_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/f3/3f3e_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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==See Also== | ==See Also== | ||
*[[Leucine transporter|Leucine transporter]] | *[[Leucine transporter|Leucine transporter]] | ||
*[[Symporter|Symporter]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 01:12, 3 December 2018
Crystal structure of LeuT bound to L-leucine (30 mM) and sodiumCrystal structure of LeuT bound to L-leucine (30 mM) and sodium
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedSecondary transporters are workhorses of cellular membranes, catalyzing the movement of small molecules and ions across the bilayer and coupling substrate passage to ion gradients. However, the conformational changes that accompany substrate transport, the mechanism by which a substrate moves through the transporter, and principles of competitive inhibition remain unclear. We used crystallographic and functional studies on the leucine transporter (LeuT), a model for neurotransmitter sodium symporters, to show that various amino acid substrates induce the same occluded conformational state and that a competitive inhibitor, tryptophan (Trp), traps LeuT in an open-to-out conformation. In the Trp complex, the extracellular gate residues arginine 30 and aspartic acid 404 define a second weak binding site for substrates or inhibitors as they permeate from the extracellular solution to the primary substrate site, which demonstrates how residues that participate in gating also mediate permeation. A competitive inhibitor traps LeuT in an open-to-out conformation.,Singh SK, Piscitelli CL, Yamashita A, Gouaux E Science. 2008 Dec 12;322(5908):1655-61. PMID:19074341[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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