2z2y: Difference between revisions
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|PDB= 2z2y |SIZE=350|CAPTION= <scene name='initialview01'>2z2y</scene>, resolution 1.89Å | |PDB= 2z2y |SIZE=350|CAPTION= <scene name='initialview01'>2z2y</scene>, resolution 1.89Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene> | |LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= [http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Subtilisin Subtilisin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.21.62 3.4.21.62] </span> | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2z2x|2Z2X]], [[2z2z|2Z2Z]], [[2z30|2Z30]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2z2y FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2z2y OCA], [http://www.ebi.ac.uk/pdbsum/2z2y PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2z2y RCSB]</span> | |||
}} | }} | ||
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[[Category: Takano, K.]] | [[Category: Takano, K.]] | ||
[[Category: Tanaka, S.]] | [[Category: Tanaka, S.]] | ||
[[Category: autoprocessed]] | [[Category: autoprocessed]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
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[[Category: thermococcus kodakaraensis]] | [[Category: thermococcus kodakaraensis]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:17:56 2008'' | ||
Revision as of 05:17, 31 March 2008
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| , resolution 1.89Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Subtilisin, with EC number 3.4.21.62 | ||||||
| Related: | 2Z2X, 2Z2Z, 2Z30
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of autoprocessed form of Tk-subtilisin
OverviewOverview
Subtilisin from the hyperthermophilic archaeon Thermococcus kodakaraensis (Tk-subtilisin) is matured from Pro-Tk-subtilisin upon autoprocessing and degradation of the propeptide. The crystal structures of the autoprocessed and mature forms of Tk-subtilisin were determined at 1.89 A and 1.70 A resolution, respectively. Comparison of these structures with that of unautoprocessed Pro-Tk-subtilisin indicates that the structure of Tk-subtilisin is not seriously changed during maturation. However, one unique Ca(2+)-binding site (Ca-7) is identified in these structures. In addition, the N-terminal region of the mature domain (Gly70-Pro82), which binds tightly to the main body in the unautoprocessed form, is disordered and mostly truncated in the autoprocessed and mature forms, respectively. Interestingly, this site is formed also in the unautoprocessed form when its crystals are soaked with 10 mM CaCl(2), as revealed by the 1.87 A structure. Along with the formation of this site, the N-terminal region (Leu75-Thr80) is disordered, with the scissile peptide bond contacting with the active site. These results indicate that the calcium ion binds weakly to the Ca-7 site in the unautoprocessed form, but is trapped upon autoprocessing. We propose that the Ca-7 site is required to promote the autoprocessing reaction by stabilizing the autoprocessed form, in which the new N terminus of the mature domain is structurally disordered. Furthermore, the crystal structure of the Tk-propeptide:S324A-subtilisin complex, which was formed by the addition of separately expressed proteins, was determined at 1.65 A resolution. This structure is virtually identical with that of the autoprocessed form, indicating that the interaction between the two domains is highly intensive and specific.
About this StructureAbout this Structure
2Z2Y is a Protein complex structure of sequences from Thermococcus kodakarensis. Full crystallographic information is available from OCA.
ReferenceReference
Four new crystal structures of Tk-subtilisin in unautoprocessed, autoprocessed and mature forms: insight into structural changes during maturation., Tanaka S, Matsumura H, Koga Y, Takano K, Kanaya S, J Mol Biol. 2007 Sep 28;372(4):1055-69. Epub 2007 Jul 26. PMID:17706669
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