Pseudouridine synthase

Pseudouridine is an abundant modified nucleotide found in RNA molecules. It is formed post-transcriptionally in RNA molecules by isomerization of the base of uridine. There are two major types of enzymes that catalyze this reaction: RNA-guided Pseudoridine synthase -- Complexes of proteins and small RNAs have been identified in archaea and eukaryotes to catalyze these reactions. The small RNA in the complex serves as a guide element to specify the site of modification through base-pairing with the target RNA molecule. Guide-independent Pseudouridine synthase -- The alternative to using the RNA-guided mechanism for pseudouridine formation is a guide-independent mechanism acting through stand-alone enzymes to catalyze formation. Structural highlights The conversion of uridine to pseudouridine causes conformational change of PUS. (1ze2).

spinqualitylabelsall models tRNA pseudouridine synthase complex with RNA and pseudouridine monophosphate 1ze2 Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image