2v1a: Difference between revisions
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|PDB= 2v1a |SIZE=350|CAPTION= <scene name='initialview01'>2v1a</scene>, resolution 1.65Å | |PDB= 2v1a |SIZE=350|CAPTION= <scene name='initialview01'>2v1a</scene>, resolution 1.65Å | ||
|SITE= <scene name='pdbsite=AC1:Fmn+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Gol+Binding+Site+For+Chain+A'>AC2</scene> and <scene name='pdbsite=AC3:Gol+Binding+Site+For+Chain+A'>AC3</scene> | |SITE= <scene name='pdbsite=AC1:Fmn+Binding+Site+For+Chain+A'>AC1</scene>, <scene name='pdbsite=AC2:Gol+Binding+Site+For+Chain+A'>AC2</scene> and <scene name='pdbsite=AC3:Gol+Binding+Site+For+Chain+A'>AC3</scene> | ||
|LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene> | |LIGAND= <scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2v0u|2V0U]], [[2v0w|2V0W]], [[2v1b|2V1B]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2v1a FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2v1a OCA], [http://www.ebi.ac.uk/pdbsum/2v1a PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2v1a RCSB]</span> | |||
}} | }} | ||
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[[Category: Halavaty, A S.]] | [[Category: Halavaty, A S.]] | ||
[[Category: Moffat, K.]] | [[Category: Moffat, K.]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: avena sativa]] | [[Category: avena sativa]] | ||
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[[Category: transferase]] | [[Category: transferase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:07:21 2008'' | ||
Revision as of 05:07, 31 March 2008
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| , resolution 1.65Å | |||||||
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| Sites: | , and | ||||||
| Ligands: | , | ||||||
| Related: | 2V0U, 2V0W, 2V1B
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
N- AND C-TERMINAL HELICES OF OAT LOV2 (404-546) ARE INVOLVED IN LIGHT-INDUCED SIGNAL TRANSDUCTION (ROOM TEMPERATURE (293K) DARK STRUCTURE OF LOV2 (404-546))
OverviewOverview
Light sensing by photoreceptors controls phototropism, chloroplast movement, stomatal opening, and leaf expansion in plants. Understanding the molecular mechanism by which these processes are regulated requires a quantitative description of photoreceptor dynamics. We focus on a light-driven signal transduction mechanism in the LOV2 domain (LOV, light, oxygen, voltage) of the blue light photoreceptor phototropin 1 from Avena sativa (oat). High-resolution crystal structures of the dark and light states of an oat LOV2 construct including residues Leu404 through Leu546 (LOV2 (404-546)) have been determined at 105 and 293 K. In all four structures, LOV2 (404-546) exhibits the typical Per-ARNT-Sim (PAS) fold, flanked by an additional conserved N-terminal turn-helix-turn motif and a C-terminal flanking region containing an amphipathic Jalpha helix. These regions dock on the LOV2 core domain and bury several hydrophobic residues of the central beta-sheet of the core domain that would otherwise be exposed to solvent. Light structures of LOV2 (404-546) reveal that formation of the covalent bond between Cys450 and the C4a atom of the flavin mononucleotide (FMN) results in local rearrangement of the hydrogen-bonding network in the FMN binding pocket. These rearrangements are associated with disruption of the Asn414-Asp515 hydrogen bond on the surface of the protein and displacement of the N- and C-terminal flanking regions of LOV2 (404-546), both of which constitute a structural signal.
About this StructureAbout this Structure
2V1A is a Single protein structure of sequence from Avena sativa. Full crystallographic information is available from OCA.
ReferenceReference
N- and C-terminal flanking regions modulate light-induced signal transduction in the LOV2 domain of the blue light sensor phototropin 1 from Avena sativa., Halavaty AS, Moffat K, Biochemistry. 2007 Dec 11;46(49):14001-9. Epub 2007 Nov 15. PMID:18001137
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