2uxk: Difference between revisions
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|PDB= 2uxk |SIZE=350|CAPTION= <scene name='initialview01'>2uxk</scene>, resolution 2.31Å | |PDB= 2uxk |SIZE=350|CAPTION= <scene name='initialview01'>2uxk</scene>, resolution 2.31Å | ||
|SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+H'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:Gol+Binding+Site+For+Chain+H'>AC1</scene> | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=CDN:CARDIOLIPIN'>CDN</scene>, <scene name='pdbligand=FE:FE+(III)+ION'>FE</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=HTO:HEPTANE-1,2,3-TRIOL'>HTO</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene>, <scene name='pdbligand=SPO:SPHEROIDENE'>SPO</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene>, <scene name='pdbligand=UQ2:UBIQUINONE-2'>UQ2</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1aig|1AIG]], [[1aij|1AIJ]], [[1ds8|1DS8]], [[1dv3|1DV3]], [[1dv6|1DV6]], [[1e14|1E14]], [[1e6d|1E6D]], [[1f6n|1F6N]], [[1fnp|1FNP]], [[1fnq|1FNQ]], [[1jgw|1JGW]], [[1jgx|1JGX]], [[1jgy|1JGY]], [[1jgz|1JGZ]], [[1jh0|1JH0]], [[1k6l|1K6L]], [[1k6n|1K6N]], [[1kby|1KBY]], [[1l9b|1L9B]], [[1l9j|1L9J]], [[1m3x|1M3X]], [[1mps|1MPS]], [[1ogv|1OGV]], [[1pcr|1PCR]], [[1pss|1PSS]], [[1pst|1PST]], [[1qov|1QOV]], [[1rg5|1RG5]], [[1rgn|1RGN]], [[1rqk|1RQK]], [[1rvj|1RVJ]], [[1ry5|1RY5]], [[1rzh|1RZH]], [[1rzz|1RZZ]], [[1s00|1S00]], [[1umx|1UMX]], [[1yst|1YST]], [[1z9j|1Z9J]], [[1z9k|1Z9K]], [[2bnp|2BNP]], [[2bns|2BNS]], [[2boz|2BOZ]], [[2gmr|2GMR]], [[2j8c|2J8C]], [[2j8d|2J8D]], [[2rcr|2RCR]], [[2uws|2UWS]], [[2uwt|2UWT]], [[2uwu|2UWU]], [[2uwv|2UWV]], [[2uww|2UWW]], [[2ux3|2UX3]], [[2ux4|2UX4]], [[2ux5|2UX5]], [[4rcr|4RCR]], [[2uxj|2UXJ]], [[2uxl|2UXL]], [[2uxm|2UXM]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uxk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uxk OCA], [http://www.ebi.ac.uk/pdbsum/2uxk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2uxk RCSB]</span> | |||
}} | }} | ||
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[[Category: Fritzsch, G.]] | [[Category: Fritzsch, G.]] | ||
[[Category: Koepke, J.]] | [[Category: Koepke, J.]] | ||
[[Category: bacteriochlorophyll]] | [[Category: bacteriochlorophyll]] | ||
[[Category: binding positions of the secondary quinone qb]] | [[Category: binding positions of the secondary quinone qb]] | ||
| Line 52: | Line 44: | ||
[[Category: transport]] | [[Category: transport]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:05:57 2008'' | ||
Revision as of 05:05, 31 March 2008
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| , resolution 2.31Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , , , , , , , , , | ||||||
| Related: | 1AIG, 1AIJ, 1DS8, 1DV3, 1DV6, 1E14, 1E6D, 1F6N, 1FNP, 1FNQ, 1JGW, 1JGX, 1JGY, 1JGZ, 1JH0, 1K6L, 1K6N, 1KBY, 1L9B, 1L9J, 1M3X, 1MPS, 1OGV, 1PCR, 1PSS, 1PST, 1QOV, 1RG5, 1RGN, 1RQK, 1RVJ, 1RY5, 1RZH, 1RZZ, 1S00, 1UMX, 1YST, 1Z9J, 1Z9K, 2BNP, 2BNS, 2BOZ, 2GMR, 2J8C, 2J8D, 2RCR, 2UWS, 2UWT, 2UWU, 2UWV, 2UWW, 2UX3, 2UX4, 2UX5, 4RCR, 2UXJ, 2UXL, 2UXM
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-RAY HIGH RESOLUTION STRUCTURE OF THE PHOTOSYNTHETIC REACTION CENTER FROM RB. SPHAEROIDES AT PH 10 IN THE CHARGE-SEPARATED STATE
OverviewOverview
The structure of the photosynthetic reaction-center from Rhodobacter sphaeroides has been determined at four different pH values (6.5, 8.0, 9.0, 10.0) in the neutral and in charge separated states. At pH 8.0, in the neutral state, we obtain a resolution of 1.87 A, which is the best ever reported for the bacterial reaction center protein. Our crystallographic data confirm the existence of two different binding positions of the secondary quinone (QB). We observe a new orientation of QB in its distal position, which shows no ring-flip compared to the orientation in the proximal position. Datasets collected for the different pH values show a pH-dependence of the population of the proximal position. The new orientation of QB in the distal position and the pH-dependence could be confirmed by continuum electrostatics calculations. Our calculations are in agreement with the experimentally observed proton uptake upon charge separation. The high resolution of our crystallographic data allows us to identify new water molecules and external residues being involved in two previously described hydrogen bond proton channels. These extended proton-transfer pathways, ending at either of the two oxo-groups of QB in its proximal position, provide additional evidence that ring-flipping is not required for complete protonation of QB upon reduction.
About this StructureAbout this Structure
2UXK is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.
ReferenceReference
pH modulates the quinone position in the photosynthetic reaction center from Rhodobacter sphaeroides in the neutral and charge separated states., Koepke J, Krammer EM, Klingen AR, Sebban P, Ullmann GM, Fritzsch G, J Mol Biol. 2007 Aug 10;371(2):396-409. Epub 2007 May 10. PMID:17570397
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Protein complex
- Rhodobacter sphaeroides
- Diehm, R.
- Fritzsch, G.
- Koepke, J.
- Bacteriochlorophyll
- Binding positions of the secondary quinone qb
- Cardiolipin
- Chlorophyll
- Chromophore
- Electron transport
- Iron
- Magnesium
- Membrane
- Metal-binding
- Photosynthesis
- Proton translocation pathway
- Reaction center
- Transmembrane
- Transport