2uu9: Difference between revisions
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|PDB= 2uu9 |SIZE=350|CAPTION= <scene name='initialview01'>2uu9</scene>, resolution 3.10Å | |PDB= 2uu9 |SIZE=350|CAPTION= <scene name='initialview01'>2uu9</scene>, resolution 3.10Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=6MZ:N6-METHYLADENOSINE-5'-MONOPHOSPHATE'>6MZ</scene>, <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=CM0:5-(CARBOXYMETHOXY)+URIDINE-5'-MONOPHOSPHATE'>CM0</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PAR:PAROMOMYCIN'>PAR</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY=[[1fjg|1FJG]], [[1gix|1GIX]], [[1i94|1I94]], [[1i95|1I95]], [[1i96|1I96]], [[1i97|1I97]], [[1ibk|1IBK]], [[1ibl|1IBL]], [[1ibm|1IBM]], [[1j5e|1J5E]], [[1jgo|1JGO]], [[1jgp|1JGP]], [[1jgq|1JGQ]], [[1l1u|1L1U]], [[1n32|1N32]], [[1n33|1N33]], [[1n34|1N34]], [[1n36|1N36]], [[1pns|1PNS]], [[1pnx|1PNX]], [[1xmo|1XMO]], [[1xmq|1XMQ]], [[1xnq|1XNQ]], [[1xnr|1XNR]], [[1yl4|1YL4]], [[2b64|2B64]], [[2b9m|2B9M]], [[2b9o|2B9O]], [[2f4v|2F4V]], [[2j00|2J00]], [[2j02|2J02]], [[2uua|2UUA]], [[2uub|2UUB]], [[2uuc|2UUC]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uu9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uu9 OCA], [http://www.ebi.ac.uk/pdbsum/2uu9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2uu9 RCSB]</span> | |||
}} | }} | ||
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[[Category: Vendeix, F A.P.]] | [[Category: Vendeix, F A.P.]] | ||
[[Category: Weixlbaumer, A.]] | [[Category: Weixlbaumer, A.]] | ||
[[Category: cmo5u]] | [[Category: cmo5u]] | ||
[[Category: coiled coil]] | [[Category: coiled coil]] | ||
| Line 50: | Line 49: | ||
[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:04:31 2008'' | ||
Revision as of 05:04, 31 March 2008
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| , resolution 3.10Å | |||||||
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| Ligands: | , , , , , , , , , | ||||||
| Related: | 1FJG, 1GIX, 1I94, 1I95, 1I96, 1I97, 1IBK, 1IBL, 1IBM, 1J5E, 1JGO, 1JGP, 1JGQ, 1L1U, 1N32, 1N33, 1N34, 1N36, 1PNS, 1PNX, 1XMO, 1XMQ, 1XNQ, 1XNR, 1YL4, 2B64, 2B9M, 2B9O, 2F4V, 2J00, 2J02, 2UUA, 2UUB, 2UUC
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF THE THERMUS THERMOPHILUS 30S RIBOSOMAL SUBUNIT COMPLEXED WITH A VALINE-ASL WITH CMO5U IN POSITION 34 BOUND TO AN MRNA WITH A GUG-CODON IN THE A-SITE AND PAROMOMYCIN.
OverviewOverview
One of the most prevalent base modifications involved in decoding is uridine 5-oxyacetic acid at the wobble position of tRNA. It has been known for several decades that this modification enables a single tRNA to decode all four codons in a degenerate codon box. We have determined structures of an anticodon stem-loop of tRNA(Val) containing the modified uridine with all four valine codons in the decoding site of the 30S ribosomal subunit. An intramolecular hydrogen bond involving the modification helps to prestructure the anticodon loop. We found unusual base pairs with the three noncomplementary codon bases, including a G.U base pair in standard Watson-Crick geometry, which presumably involves an enol form for the uridine. These structures suggest how a modification in the uridine at the wobble position can expand the decoding capability of a tRNA.
About this StructureAbout this Structure
2UU9 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
ReferenceReference
Mechanism for expanding the decoding capacity of transfer RNAs by modification of uridines., Weixlbaumer A, Murphy FV 4th, Dziergowska A, Malkiewicz A, Vendeix FA, Agris PF, Ramakrishnan V, Nat Struct Mol Biol. 2007 Jun;14(6):498-502. Epub 2007 May 13. PMID:17496902
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Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)
OCA- Pages with broken file links
- Protein complex
- Thermus thermophilus
- Agris, P F.
- Dziergowska, A.
- Malkiewicz, A.
- Murphy, F V.
- Ramakrishnan, V.
- Vendeix, F A.P.
- Weixlbaumer, A.
- Cmo5u
- Coiled coil
- Metal-binding
- Modifiaction
- Mrna
- Paromomycin
- Ribonucleoprotein
- Ribosomal protein
- Ribosome
- Rna-binding
- Rrna-binding
- Translation
- Trna
- Trna-binding
- Zinc
- Zinc-finger