2rhx: Difference between revisions
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|PDB= 2rhx |SIZE=350|CAPTION= <scene name='initialview01'>2rhx</scene>, resolution 2.1Å | |PDB= 2rhx |SIZE=350|CAPTION= <scene name='initialview01'>2rhx</scene>, resolution 2.1Å | ||
|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand= | |LIGAND= <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= L3MBTL, KIAA0681, L3MBT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= L3MBTL, KIAA0681, L3MBT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
|DOMAIN= | |||
|RELATEDENTRY=[[2rhi|2RHI]], [[2rhu|2RHU]], [[2rhy|2RHY]], [[2rhz|2RHZ]], [[2ri2|2RI2]], [[2ri3|2RI3]], [[2ri5|2RI5]] | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2rhx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2rhx OCA], [http://www.ebi.ac.uk/pdbsum/2rhx PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2rhx RCSB]</span> | |||
}} | }} | ||
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[[Category: Li, H.]] | [[Category: Li, H.]] | ||
[[Category: Patel, D J.]] | [[Category: Patel, D J.]] | ||
[[Category: alternative splicing]] | [[Category: alternative splicing]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
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[[Category: zinc-finger]] | [[Category: zinc-finger]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 05:00:31 2008'' | ||
Revision as of 05:00, 31 March 2008
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| , resolution 2.1Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | L3MBTL, KIAA0681, L3MBT (Homo sapiens) | ||||||
| Related: | 2RHI, 2RHU, 2RHY, 2RHZ, 2RI2, 2RI3, 2RI5
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the 3-MBT repeats from human L3MBTL1 bound to dimethyl-lysine
OverviewOverview
Human L3MBTL1, which contains three malignant brain tumor (MBT) repeats, binds monomethylated and dimethylated lysines, but not trimethylated lysines, in several histone sequence contexts. In crystal structures of L3MBTL1 complexes, the monomethyl- and dimethyllysines insert into a narrow and deep cavity of aromatic residue-lined pocket 2, while a proline ring inserts into shallower pocket 1. We have also engineered a single Y to E substitution within the aromatic cage of the BPTF PHD finger, resulting in a reversal of binding preference from trimethyl- to dimethyllysine in an H3K4 sequence context. In both the "cavity insertion" (L3MBTL1) and "surface groove" (PHD finger) modes of methyllysine recognition, a carboxylate group both hydrogen bonds and ion pairs to the methylammonium proton. Our structural and binding studies of these two modules provide insights into the molecular principles governing the decoding of lysine methylation states, thereby highlighting a methylation state-specific layer of histone mark readout impacting on epigenetic regulation.
About this StructureAbout this Structure
2RHX is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for lower lysine methylation state-specific readout by MBT repeats of L3MBTL1 and an engineered PHD finger., Li H, Fischle W, Wang W, Duncan EM, Liang L, Murakami-Ishibe S, Allis CD, Patel DJ, Mol Cell. 2007 Nov 30;28(4):677-91. PMID:18042461
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