2zbj: Difference between revisions
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==Crystal structure of Dioclea rostrata lectin== | ==Crystal structure of Dioclea rostrata lectin== | ||
<StructureSection load='2zbj' size='340' side='right' caption='[[2zbj]], [[Resolution|resolution]] 2.05Å' scene=''> | <StructureSection load='2zbj' size='340' side='right' caption='[[2zbj]], [[Resolution|resolution]] 2.05Å' scene=''> | ||
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<table><tr><td colspan='2'>[[2zbj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioclea_rostrata Dioclea rostrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZBJ FirstGlance]. <br> | <table><tr><td colspan='2'>[[2zbj]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Dioclea_rostrata Dioclea rostrata]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2ZBJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ZBJ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbj OCA], [http://pdbe.org/2zbj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zbj RCSB], [http://www.ebi.ac.uk/pdbsum/2zbj PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2zbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2zbj OCA], [http://pdbe.org/2zbj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2zbj RCSB], [http://www.ebi.ac.uk/pdbsum/2zbj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2zbj ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/2zbj_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/zb/2zbj_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
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[[Category: Rocha, B A.M da]] | [[Category: Rocha, B A.M da]] | ||
[[Category: Sousa, E P.de]] | [[Category: Sousa, E P.de]] | ||
[[Category: Calcium]] | |||
[[Category: Diocleinae]] | [[Category: Diocleinae]] | ||
[[Category: Lectin]] | [[Category: Lectin]] | ||
Revision as of 12:24, 17 October 2018
Crystal structure of Dioclea rostrata lectinCrystal structure of Dioclea rostrata lectin
Structural highlights
Function[LECA_DIORO] D-mannose/D-glucose-binding lectin. Induces histamine release in mast cells from hamster and rat. Induces lymphocyte proliferation and IFNG production.[1] [2] [3] [4] [5] [6] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe legume lectins from the subtribe Diocleinae, often referred to as concanavalin A-like lectins, are a typical example of highly similar proteins that show distinct biological activities. The pH-dependent oligomerization that some of these lectins undergo and the relative position of amino acids within the carbohydrate-binding site are factors that have been reported to contribute to these differences in the activities of Diocleinae lectins. In the present work, we determined the amino acid sequence and the crystal structure of the lectin of Dioclea rostrata seeds (DRL), with the aim of investigating the structural bases of the different behavior displayed by this lectin in comparison to other Diocleinae lectins and determining the reason for the distinct pH-dependent dimer-tetramer equilibrium. In addition, we discovered a novel multimeric arrangement for this lectin. Crystal structure of Dioclea rostrata lectin: insights into understanding the pH-dependent dimer-tetramer equilibrium and the structural basis for carbohydrate recognition in Diocleinae lectins.,de Oliveira TM, Delatorre P, da Rocha BA, de Souza EP, Nascimento KS, Bezerra GA, Moura TR, Benevides RG, Bezerra EH, Moreno FB, Freire VN, de Azevedo WF Jr, Cavada BS J Struct Biol. 2008 Nov;164(2):177-82. Epub 2008 Jul 22. PMID:18682294[7] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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