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</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vtd OCA], [http://pdbe.org/1vtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vtd RCSB], [http://www.ebi.ac.uk/pdbsum/1vtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vtd ProSAT]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1vtd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1vtd OCA], [http://pdbe.org/1vtd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1vtd RCSB], [http://www.ebi.ac.uk/pdbsum/1vtd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1vtd ProSAT]</span></td></tr> | ||
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== Publication Abstract from PubMed == | |||
The target sequence of the restriction enzyme NarI (GGCGCC) is a hot spot for the -2 frameshift mutagenesis (GGCGCC----GGCC) induced by the chemical carcinogens such as N-2-acetyl-aminofluorene. Of the guanine residues, all of which show equal reactivity towards the carcinogen, only binding to the 3'-most proximal guanine within the NarI site is able to trigger the frameshift event. We selected the non-palindromic dodecamer d(ACCGGCGCCACA), whose sequence corresponds to the most mutagenic NarI site in pBR322 DNA; for X-ray structure analysis. Its molecular structure determined at 2.8 A resolution reveals significant deviations from the structure of canonical B-form DNA, with partial opening of three G-C base pairs, high propeller twist values and sequence-dependent three-centred hydrogen bonds. This crystal structure shows a novel kind of packing in which helices are locked together by groove-backbone interactions. The partial opening of G-C base pairs is induced by interactions of phosphate anionic oxygen atoms with the amino group of cytosine bases. This provides a model for close approach of DNA molecules during biological processes, such as recombination. | |||
Unusual helical packing in crystals of DNA bearing a mutation hot spot.,Timsit Y, Westhof E, Fuchs RP, Moras D Nature. 1989 Oct 5;341(6241):459-62. doi: 10.1038/341459a0. PMID:2797169<ref>PMID:2797169</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
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<div class="pdbe-citations 1vtd" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 11:17, 10 October 2018
UNUSUAL HELICAL PACKING IN CRYSTALS OF DNA BEARING A MUTATION HOT SPOTUNUSUAL HELICAL PACKING IN CRYSTALS OF DNA BEARING A MUTATION HOT SPOT
Structural highlights
Publication Abstract from PubMedThe target sequence of the restriction enzyme NarI (GGCGCC) is a hot spot for the -2 frameshift mutagenesis (GGCGCC----GGCC) induced by the chemical carcinogens such as N-2-acetyl-aminofluorene. Of the guanine residues, all of which show equal reactivity towards the carcinogen, only binding to the 3'-most proximal guanine within the NarI site is able to trigger the frameshift event. We selected the non-palindromic dodecamer d(ACCGGCGCCACA), whose sequence corresponds to the most mutagenic NarI site in pBR322 DNA; for X-ray structure analysis. Its molecular structure determined at 2.8 A resolution reveals significant deviations from the structure of canonical B-form DNA, with partial opening of three G-C base pairs, high propeller twist values and sequence-dependent three-centred hydrogen bonds. This crystal structure shows a novel kind of packing in which helices are locked together by groove-backbone interactions. The partial opening of G-C base pairs is induced by interactions of phosphate anionic oxygen atoms with the amino group of cytosine bases. This provides a model for close approach of DNA molecules during biological processes, such as recombination. Unusual helical packing in crystals of DNA bearing a mutation hot spot.,Timsit Y, Westhof E, Fuchs RP, Moras D Nature. 1989 Oct 5;341(6241):459-62. doi: 10.1038/341459a0. PMID:2797169[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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