2xdy: Difference between revisions
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== | ==Crystal structure of the N. crassa QDE-2 AGO MID domain== | ||
<StructureSection load='2xdy' size='340' side='right' caption='[[2xdy]], [[Resolution|resolution]] 2.20Å' scene=''> | <StructureSection load='2xdy' size='340' side='right' caption='[[2xdy]], [[Resolution|resolution]] 2.20Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
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Check<jmol> | Check<jmol> | ||
<jmolCheckbox> | <jmolCheckbox> | ||
<scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xd/2xdy_consurf.spt"</scriptWhenChecked> | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xd/2xdy_consurf.spt"</scriptWhenChecked> | ||
<scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> | ||
<text>to colour the structure by Evolutionary Conservation</text> | <text>to colour the structure by Evolutionary Conservation</text> | ||
Revision as of 12:26, 3 October 2018
Crystal structure of the N. crassa QDE-2 AGO MID domainCrystal structure of the N. crassa QDE-2 AGO MID domain
Structural highlights
Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedArgonaute (AGO) proteins are core components of RNA-induced silencing complexes and have essential roles in RNA-mediated gene silencing. They are characterized by a bilobal architecture, consisting of one lobe containing the amino-terminal and PAZ domains and another containing the MID and PIWI domains. Except for the PAZ domain, structural information on eukaryotic AGO domains is not yet available. In this study, we report the crystal structure of the MID domain of the eukaryotic AGO protein QDE-2 from Neurospora crassa. This domain adopts a Rossmann-like fold and recognizes the 5'-terminal nucleotide of a guide RNA in a manner similar to its prokaryotic counterparts. The 5'-nucleotide-binding site shares common residues with a second, adjacent ligand-binding site, suggesting a mechanism for the cooperative binding of ligands to the MID domain of eukaryotic AGOs. Crystal structure and ligand binding of the MID domain of a eukaryotic Argonaute protein.,Boland A, Tritschler F, Heimstadt S, Izaurralde E, Weichenrieder O EMBO Rep. 2010 Jul;11(7):522-7. Epub 2010 Jun 11. PMID:20539312[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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