2qkl: Difference between revisions
New page: left|200px {{Structure |PDB= 2qkl |SIZE=350|CAPTION= <scene name='initialview01'>2qkl</scene>, resolution 2.330Å |SITE= |LIGAND= <scene name='pdbligand=PB:LEAD+... |
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|GENE= dcp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), SPAC19A8.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]) | |GENE= dcp1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]), SPAC19A8.12 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4896 Schizosaccharomyces pombe]) | ||
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam06058 DCP1], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam05026 DCP2]</span> | |DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam06058 DCP1], [http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=pfam05026 DCP2]</span> | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qkl OCA], [http://www.ebi.ac.uk/pdbsum/2qkl PDBsum | |RELATEDENTRY= | ||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2qkl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2qkl OCA], [http://www.ebi.ac.uk/pdbsum/2qkl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2qkl RCSB]</span> | |||
}} | }} | ||
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[[Category: protein-protein complex]] | [[Category: protein-protein complex]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:50:24 2008'' | ||
Revision as of 04:50, 31 March 2008
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| , resolution 2.330Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | dcp1 (Schizosaccharomyces pombe), SPAC19A8.12 (Schizosaccharomyces pombe) | ||||||
| Activity: | M(7)G(5')pppN diphosphatase, with EC number 3.6.1.30 | ||||||
| Domains: | DCP1, DCP2 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
The crystal structure of fission yeast mRNA decapping enzyme Dcp1-Dcp2 complex
OverviewOverview
A critical step in mRNA degradation is the removal of the 5' cap structure, which is catalyzed by the Dcp1-Dcp2 complex. The crystal structure of an S. pombe Dcp1p-Dcp2n complex combined with small-angle X-ray scattering analysis (SAXS) reveals that Dcp2p exists in open and closed conformations, with the closed complex being, or closely resembling, the catalytically more active form. This suggests that a conformational change between these open and closed complexes might control decapping. A bipartite RNA-binding channel containing the catalytic site and Box B motif is identified with a bound ATP located in the catalytic pocket in the closed complex, suggesting possible interactions that facilitate substrate binding. Dcp1 stimulates the activity of Dcp2 by promoting and/or stabilizing the closed complex. Notably, the interface of Dcp1 and Dcp2 is not fully conserved, explaining why the Dcp1-Dcp2 interaction in higher eukaryotes requires an additional factor.
About this StructureAbout this Structure
2QKL is a Protein complex structure of sequences from Schizosaccharomyces pombe. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis of dcp2 recognition and activation by dcp1., She M, Decker CJ, Svergun DI, Round A, Chen N, Muhlrad D, Parker R, Song H, Mol Cell. 2008 Feb 15;29(3):337-49. PMID:18280239
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