Ubiquitin conjugating enzyme: Difference between revisions
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'''Ubiquitin conjugating enzyme''' (Ubc) or '''E2 enzyme''' catalyzes the second step in the ubiquitination of a protein tagged to be degraded by the proteasome. Ubiquitin activating enzyme (E1) and ATP produce a C-terminal acyl adenylated ubiquitin molecule which binds to ubiquitin conjugating enzyme (E2) (Ubc) cysteine<ref>PMID:19549727</ref>. The Ubc then binds to ubiquitin ligase (E3) via a conserved binding region. E3 catalyzes the transfer of ubiquitin from the Ubc-ubiquitin complex to a lysine residue of the target protein. Ubc13 makes a catalytically active heterodimer with MMS2<ref>PMID:16980971</ref>.<br /> | '''Ubiquitin conjugating enzyme''' (Ubc) or '''E2 enzyme''' catalyzes the second step in the ubiquitination of a protein tagged to be degraded by the proteasome. Ubiquitin activating enzyme (E1) and ATP produce a C-terminal acyl adenylated ubiquitin molecule which binds to ubiquitin conjugating enzyme (E2) (Ubc) cysteine<ref>PMID:19549727</ref>. The Ubc then binds to ubiquitin ligase (E3) via a conserved binding region. E3 catalyzes the transfer of ubiquitin from the Ubc-ubiquitin complex to a lysine residue of the target protein. Ubc13 makes a catalytically active heterodimer with MMS2<ref>PMID:16980971</ref>.<br /> | ||
For more details on Ubc13 see [[UBC13 MMS2]].<br /> | *'''UBC2''' is required for silencing in yeast<ref>PMID:9343433</ref>.<br /> | ||
For more details on Ubc9 see [[Ubc9]].<br /> | *'''UBC4 UBC5''' mediate selective degradation of short-lived and abnormal proteins<ref>PMID:2154373</ref>.<br /> | ||
*'''UBC6''' participates in ER-associated protein degradation<ref>PMID:12082160</ref>.<br /> | |||
*'''UBC7 or UBC13''' is key in the process of tagging target proteins with Lys63-linked polyubiquitin<ref>PMID:16862162</ref>.<br /> For more details on Ubc13 see [[UBC13 MMS2]].<br /> | |||
*'''UBC8''' is interferon-inducible<ref>PMID:15485925</ref>.<br /> | |||
*'''UBC9''' is required for sumoylation<ref>PMID:24706591</ref>.<br /> For more details on Ubc9 see [[SUMO conjugating enzyme Ubc9]].<br /> | |||
*'''UBC16''' conjugates ubiquitin to its N-terminus and to that of the small ubiquitin-like modifier SUMO<ref>PMID:23560854</ref>.<br /> | |||
*'''UBC D3''' functions in the ubiquitination of the tumor suppressor p53.<br /> | |||
==Medical importance== | |||
UBC13 is required for metastatic spread and lung colonizationin breast cancer<ref>PMID:25189770</ref>.<br /> | |||
== Structural highlights == | == Structural highlights == | ||
The active site of Ubc contains a cysteine residue. The <scene name='55/551219/Cv/2'>ubiquitin surface which interacts with Ubc</scene><ref>PMID:21396940</ref>. | The active site of Ubc contains a cysteine residue. The <scene name='55/551219/Cv/2'>ubiquitin surface which interacts with Ubc</scene><ref>PMID:21396940</ref>. | ||