6faq: Difference between revisions
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<StructureSection load='6faq' size='340' side='right' caption='[[6faq]], [[Resolution|resolution]] 1.95Å' scene=''> | <StructureSection load='6faq' size='340' side='right' caption='[[6faq]], [[Resolution|resolution]] 1.95Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6faq]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAQ FirstGlance]. <br> | <table><tr><td colspan='2'>[[6faq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Halsa Halsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAQ FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BR:BROMIDE+ION'>BR</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VNG_0258H ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64091 HALSA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6faq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6faq OCA], [http://pdbe.org/6faq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6faq RCSB], [http://www.ebi.ac.uk/pdbsum/6faq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6faq ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6faq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6faq OCA], [http://pdbe.org/6faq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6faq RCSB], [http://www.ebi.ac.uk/pdbsum/6faq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6faq ProSAT]</span></td></tr> | ||
</table> | </table> | ||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein-DNA interactions are highly dependent on salt concentration. To gain insight into how such interactions are maintained in the highly saline cytoplasm of halophilic archaea, we determined the 3-D structure of VNG0258H/RosR, the first haloarchaeal DNA-binding protein from the extreme halophilic archaeon Halobactrium salinarum. It is a dimeric winged-helix-turn-helix (wHTH) protein with unique features due to adaptation to the halophilic environment. As ions are major players in DNA binding processes, particularly in halophilic environments, we investigated the solution structure of the ionic envelope and located anions in the first shell around the protein in the crystal using anomalous scattering. Anions that were found to be tightly bound to residues in the positively charged DNA-binding site would probably be released upon DNA binding and will thus make significant contribution to the driving force of the binding process. Unexpectedly, ions were also found in a buried internal cavity connected to the external medium by a tunnel. Our structure lays a solid groundwork for future structural, computational and biochemical studies on complexes of the protein with cognate DNA sequences, with implications to protein-DNA interactions in hyper-saline environments. | |||
The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.,Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B J Struct Biol. 2018 Aug 12. pii: S1047-8477(18)30226-0. doi:, 10.1016/j.jsb.2018.08.008. PMID:30110657<ref>PMID:30110657</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 6faq" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Halsa]] | |||
[[Category: Kutnowski, N]] | [[Category: Kutnowski, N]] | ||
[[Category: Shaanan, B]] | [[Category: Shaanan, B]] | ||
Revision as of 11:00, 29 August 2018
Structure of H. salinarum RosR (vng0258) grown from KBrStructure of H. salinarum RosR (vng0258) grown from KBr
Structural highlights
Publication Abstract from PubMedProtein-DNA interactions are highly dependent on salt concentration. To gain insight into how such interactions are maintained in the highly saline cytoplasm of halophilic archaea, we determined the 3-D structure of VNG0258H/RosR, the first haloarchaeal DNA-binding protein from the extreme halophilic archaeon Halobactrium salinarum. It is a dimeric winged-helix-turn-helix (wHTH) protein with unique features due to adaptation to the halophilic environment. As ions are major players in DNA binding processes, particularly in halophilic environments, we investigated the solution structure of the ionic envelope and located anions in the first shell around the protein in the crystal using anomalous scattering. Anions that were found to be tightly bound to residues in the positively charged DNA-binding site would probably be released upon DNA binding and will thus make significant contribution to the driving force of the binding process. Unexpectedly, ions were also found in a buried internal cavity connected to the external medium by a tunnel. Our structure lays a solid groundwork for future structural, computational and biochemical studies on complexes of the protein with cognate DNA sequences, with implications to protein-DNA interactions in hyper-saline environments. The 3-D structure of VNG0258H/RosR - A haloarchaeal DNA-binding protein in its ionic shell.,Kutnowski N, Shmuely H, Dahan I, Shmulevich F, Davidov G, Shahar A, Eichler J, Zarivach R, Shaanan B J Struct Biol. 2018 Aug 12. pii: S1047-8477(18)30226-0. doi:, 10.1016/j.jsb.2018.08.008. PMID:30110657[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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