CRISPR-Cas9: Difference between revisions

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 '''Recognition Mechanism of the sgRNA Scaffold'''
-The <scene name='74/742625/Cv6/31'>repeat:anti-repeat duplex is recognized by the REC and WED domains, primarily through interactions between the protein and the sugar-phosphate backbone</scene>. Consistent with our data showing that the distorted repeat:anti-repeat duplex is critical for Cas9-catalyzed DNA cleavage, the internal loop is recognized by the WED domain. The 2'-OH of C30 hydrogen bonds with Tyr868, and the backbone phosphate groups of U31, C45, and U46 interact with Lys870, Arg792, and Lys881, respectively. These structural observations explain the structure-dependent recognition of the repeat:anti-repeat duplex by SaCas9.
+The <scene name='74/742625/Cv6/32'>repeat:anti-repeat duplex is recognized by the REC and WED domains, primarily through interactions between the protein and the sugar-phosphate backbone</scene>. Consistent with our data showing that the distorted repeat:anti-repeat duplex is critical for Cas9-catalyzed DNA cleavage, the internal loop is recognized by the WED domain. The 2'-OH of C30 hydrogen bonds with Tyr868, and the backbone phosphate groups of U31, C45, and U46 interact with Lys870, Arg792, and Lys881, respectively. These structural observations explain the structure-dependent recognition of the repeat:anti-repeat duplex by SaCas9.
 =See aslo=
 *[[Cas9]]