2p6r: Difference between revisions
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|PDB= 2p6r |SIZE=350|CAPTION= <scene name='initialview01'>2p6r</scene>, resolution 3.000Å | |PDB= 2p6r |SIZE=350|CAPTION= <scene name='initialview01'>2p6r</scene>, resolution 3.000Å | ||
|SITE= | |SITE= | ||
|LIGAND= | |LIGAND= <scene name='pdbligand=DA:2'-DEOXYADENOSINE-5'-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2'-DEOXYCYTIDINE-5'-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5'-MONOPHOSPHATE'>DT</scene> | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
|DOMAIN= | |||
|RELATEDENTRY= | |||
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2p6r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2p6r OCA], [http://www.ebi.ac.uk/pdbsum/2p6r PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2p6r RCSB]</span> | |||
}} | }} | ||
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[[Category: Nehring, S.]] | [[Category: Nehring, S.]] | ||
[[Category: archaeal helicase]] | [[Category: archaeal helicase]] | ||
[[Category: dna repair]] | [[Category: dna repair,]] | ||
[[Category: protein-dna complex]] | [[Category: protein-dna complex]] | ||
[[Category: sf2 helicase]] | [[Category: sf2 helicase]] | ||
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 04:30:55 2008'' | ||
Revision as of 04:30, 31 March 2008
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| , resolution 3.000Å | |||||||
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| Ligands: | , , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of superfamily 2 helicase Hel308 in complex with unwound DNA
OverviewOverview
To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3'-->5' helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase theta. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require ATP and identifies a prominent beta-hairpin loop as the unwinding element. Similar loops in hepatitis C virus NS3 helicase and RNA-decay factors support the idea that this duplex-unwinding mechanism is applicable to a broad subset of SF2 helicases. Comparison with ATP-bound SF2 enzymes suggests that ATP promotes processive unwinding of 1 base pair by ratchet-like transport of the 3' product strand. Our results provide a first structural framework for strand separation by processive SF2 3'-->5' helicases and reveal important mechanistic differences from SF1 helicases.
About this StructureAbout this Structure
2P6R is a Protein complex structure of sequences from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
ReferenceReference
Structural basis for DNA duplex separation by a superfamily-2 helicase., Buttner K, Nehring S, Hopfner KP, Nat Struct Mol Biol. 2007 Jul;14(7):647-52. Epub 2007 Jun 10. PMID:17558417
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