CRISPR-Cas9: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
No edit summary |
||
| Line 66: | Line 66: | ||
'''Recognition Mechanism of the Guide:Target Heteroduplex''' | '''Recognition Mechanism of the Guide:Target Heteroduplex''' | ||
The <scene name='74/742625/Cv6/17'>guide:target heteroduplex is accommodated in the central channel</scene> formed between the REC and NUC lobes. The sugar-phosphate backbone of the PAM-distal region (A3–U6) of the sgRNA interacts with the REC lobe (Thr238, Tyr239, Lys248, Tyr256, Arg314, Asn394, and Gln414). | The <scene name='74/742625/Cv6/17'>guide:target heteroduplex is accommodated in the central channel</scene> formed between the REC and NUC lobes. The sugar-phosphate backbone of the PAM-distal region (A3–U6) of the sgRNA interacts with the <scene name='74/742625/Cv6/20'>REC lobe (Thr238, Tyr239, Lys248, Tyr256, Arg314, Asn394, and Gln414)</scene>. | ||
=See aslo= | =See aslo= | ||
*[[Cas9]] | *[[Cas9]] | ||