2mfa: Difference between revisions
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== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[2mfa]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MFA FirstGlance]. <br> | <table><tr><td colspan='2'>[[2mfa]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2MFA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2MFA FirstGlance]. <br> | ||
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mfa OCA], [http://pdbe.org/2mfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mfa RCSB], [http://www.ebi.ac.uk/pdbsum/2mfa PDBsum]</span></td></tr> | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2mfa FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2mfa OCA], [http://pdbe.org/2mfa PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2mfa RCSB], [http://www.ebi.ac.uk/pdbsum/2mfa PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2mfa ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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Revision as of 02:00, 10 August 2018
Mambalgin-2Mambalgin-2
Structural highlights
Publication Abstract from PubMedMambalgins are a novel class of snake venom components that exert potent analgesic effects mediated through the inhibition of acid-sensing ion channels (ASICs). The 57-residue polypeptide mambalgin-2 (Ma-2) was synthesized by using a combination of solid-phase peptide synthesis and native chemical ligation. The structure of the synthetic toxin, determined using homonuclear NMR, revealed an unusual three-finger toxin fold reminiscent of functionally unrelated snake toxins. Electrophysiological analysis of Ma-2 on wild-type and mutant ASIC1a receptors allowed us to identify alpha-helix 5, which borders on the functionally critical acidic pocket of the channel, as a major part of the Ma-2 binding site. This region is also crucial for the interaction of ASIC1a with the spider toxin PcTx1, thus suggesting that the binding sites for these toxins substantially overlap. This work lays the foundation for structure-activity relationship (SAR) studies and further development of this promising analgesic peptide. Chemical Synthesis, 3D Structure, and ASIC Binding Site of the Toxin Mambalgin-2.,Schroeder CI, Rash LD, Vila-Farres X, Rosengren KJ, Mobli M, King GF, Alewood PF, Craik DJ, Durek T Angew Chem Int Ed Engl. 2013 Dec 9. doi: 10.1002/anie.201308898. PMID:24323786[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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