2m48: Difference between revisions
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==Solution Structure of IBR-RING2 Tandem Domain from Parkin== | ==Solution Structure of IBR-RING2 Tandem Domain from Parkin== | ||
<StructureSection load='2m48' size='340' side='right' caption='[[2m48]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | <StructureSection load='2m48' size='340' side='right' caption='[[2m48]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | ||
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jmo|2jmo]], [[1wd2|1wd2]]</td></tr> | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2jmo|2jmo]], [[1wd2|1wd2]]</td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG10523, park ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CG10523, park ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m48 OCA], [http://pdbe.org/2m48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m48 RCSB], [http://www.ebi.ac.uk/pdbsum/2m48 PDBsum]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2m48 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2m48 OCA], [http://pdbe.org/2m48 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2m48 RCSB], [http://www.ebi.ac.uk/pdbsum/2m48 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2m48 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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Revision as of 01:43, 10 August 2018
Solution Structure of IBR-RING2 Tandem Domain from ParkinSolution Structure of IBR-RING2 Tandem Domain from Parkin
Structural highlights
Publication Abstract from PubMedMutations in the park2 gene, encoding the RING-inBetweenRING-RING E3 ubiquitin ligase parkin, cause 50% of autosomal recessive juvenile Parkinsonism cases. More than 70 known pathogenic mutations occur throughout parkin, many of which cluster in the inhibitory amino-terminal ubiquitin-like domain, and the carboxy-terminal RING2 domain that is indispensable for ubiquitin transfer. A structural rationale showing how autosomal recessive juvenile Parkinsonism mutations alter parkin function is still lacking. Here we show that the structure of parkin RING2 is distinct from canonical RING E3 ligases and lacks key elements required for E2-conjugating enzyme recruitment. Several pathogenic mutations in RING2 alter the environment of a single surface-exposed catalytic cysteine to inhibit ubiquitination. Native parkin adopts a globular inhibited conformation in solution facilitated by the association of the ubiquitin-like domain with the RING-inBetweenRING-RING C-terminus. Autosomal recessive juvenile Parkinsonism mutations disrupt this conformation. Finally, parkin autoubiquitinates only in cis, providing a molecular explanation for the recessive nature of autosomal recessive juvenile Parkinsonism. A molecular explanation for the recessive nature of parkin-linked Parkinson's disease.,Spratt DE, Julio Martinez-Torres R, Noh YJ, Mercier P, Manczyk N, Barber KR, Aguirre JD, Burchell L, Purkiss A, Walden H, Shaw GS Nat Commun. 2013 Jun 17;4:1983. doi: 10.1038/ncomms2983. PMID:23770917[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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