2bj8: Difference between revisions
No edit summary |
No edit summary |
||
| Line 33: | Line 33: | ||
[[Category: transcription regulation]] | [[Category: transcription regulation]] | ||
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov 5 17:40:33 2007'' | ||
Revision as of 18:35, 5 November 2007
|
NIKR IN CLOSED CONFORMATION AND NICKEL BOUND TO HIGH AND LOW-AFFINITY SITES
OverviewOverview
The Pyrococcus horikoshii OT3 genome contains a gene (PH0601 or nikR), encoding a protein (PhNikR) that shares 33.8% amino acid sequence identity, with Escherichia coli nickel responsive repressor NikR (EcNikR), including, many residues that are functionally important in the E.coli ortholog. We, succeeded in crystallization and structural characterization of PhNikR in, the apo form and two nickel bound forms that exhibit different, conformations, open and closed. Moreover, we have identified a putative, "low-affinity" nickel-binding pocket in the closed form. This binding site, has unusual nickel coordination and exhibits high sensitivity to phosphate, in the crystal structure. Analysis of the PhNikR structures and, structure-based mutational studies with EcNikR reveals a plausible, mechanism of nickel-dependent promoter recognition by the NikR family of, proteins.
About this StructureAbout this Structure
2BJ8 is a Single protein structure of sequence from Pyrococcus horikoshii with NI, CL, PG4, EPE and EDO as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.
ReferenceReference
Structure of Pyrococcus horikoshii NikR: nickel sensing and implications for the regulation of DNA recognition., Chivers PT, Tahirov TH, J Mol Biol. 2005 May 6;348(3):597-607. PMID:15826657
Page seeded by OCA on Mon Nov 5 17:40:33 2007