6dhy: Difference between revisions

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-'''Unreleased structure'''
-The entry 6dhy is ON HOLD  until Paper Publication
+==Crystallogrpahic tetramer of Zn-bound RIDC1 variant bearing two disulfide bonded cysteines==
+<StructureSection load='6dhy' size='340' side='right' caption='[[6dhy]], [[Resolution|resolution]] 2.22&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6dhy]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6DHY OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6DHY FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6dhy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6dhy OCA], [http://pdbe.org/6dhy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6dhy RCSB], [http://www.ebi.ac.uk/pdbsum/6dhy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6dhy ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/C562_ECOLX C562_ECOLX]] Electron-transport protein of unknown function.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Despite significant progress in protein design, the construction of protein assemblies that display complex functions (e.g., catalysis or allostery) remains a significant challenge. We recently reported the de novo construction of an allosteric supramolecular protein assembly (Zn-C38/C81/C96R14) in which the dissociation and binding of Zn(II) ions were coupled over a distance of 15 A to the selective hydrolytic breakage and formation of a single disulfide bond. Zn4-C38/C81/C96R14 was constructed by ZnII-templated assembly of a monomeric protein (R1, a derivative of cytochrome cb562) into a tetramer, followed by progressive incorporation of non-covalent and disulfide bonding interactions into the protein-protein interfaces to create a strained quaternary architecture. The interfacial strain thus built allowed mechanical coupling between the binding/dissociation of Zn(II) and formation/hydrolysis of a single disulfide bond (C38-C38) out of a possible six. While the earlier study provided structural evidence for the two end-states of allosteric coupling, the energetic basis for allosteric coupling and the minimal structural requirements for building this allosteric system were not understood. Toward this end, we have characterized the structures and Zn-binding properties of two related protein con-structs (C38/C96R1 and C38R1) which also possess C38-C38 disulfide bonds. In addition, we have carried out extensive molecular dy-namics (MD) simulations of C38/C81/C96R14 to understand the energetic basis for the selective cleavage of the C38-C38 disulfide bond upon Zn(II) dissociation. Our analyses reveal that the local interfacial environment around the C38-C38 bond is key to its selective cleavage, but this cleavage is only possible within the context of a stable quaternary architecture which enables structural coupling between Zn(II) coordination and the protein-protein interfaces.
-Authors: Tezcan, F.A., Churchfield, L.A.
+Determining the structural and energetic basis of allostery in a de novo designed metalloprotein assembly.,Churchfield LA, Alberstein RG, Williamson LM, Tezcan FA J Am Chem Soc. 2018 Jul 12. doi: 10.1021/jacs.8b05812. PMID:29996654<ref>PMID:29996654</ref>
-Description: Crystallogrpahic tetramer of Zn-bound RIDC1 variant bearing two disulfide bonded cysteines
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Tezcan, F.A]]
+<div class="pdbe-citations 6dhy" style="background-color:#fffaf0;"></div>
-[[Category: Churchfield, L.A]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Churchfield, L A]]
+[[Category: Tezcan, F A]]
+[[Category: 4-helix bundle]]
+[[Category: Electron transport]]
+[[Category: Engineered protein]]
+[[Category: Metalloprotein]]